Anti-Glutamine Synthetase antibody (Biotin) (ab34545)

Overview

  • Product name
    Anti-Glutamine Synthetase antibody (Biotin)
    See all Glutamine Synthetase primary antibodies
  • Description
    Goat polyclonal to Glutamine Synthetase (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, Dot blotmore details
  • Species reactivity
    Reacts with Microbia.
  • Immunogen

    Full length protein (Microbial)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15% Sodium chloride, 0.02% Potassium phosphate, 10mg/ml BSA. pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
Dot blot
  • Application notes
    Dot: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/20000. This product has been assayed against 1.0?g of Glutamine Synthetase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
    ICC/IF: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.
    Suitable for antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
    • Involvement in disease
      Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
    • Sequence similarities
      Belongs to the glutamine synthetase family.
    • Developmental stage
      Expressed during early fetal stages.
    • Cellular localization
      Cytoplasm. Mitochondrion.
    • Information by UniProt
    • Alternative names
      • cell proliferation-inducing protein 59 antibody
      • GLNA antibody
      • GLNA_HUMAN antibody
      • GLNS antibody
      • GLUL antibody
      • Glutamate ammonia ligase antibody
      • Glutamate decarboxylase antibody
      • Glutamate--ammonia ligase antibody
      • glutamine synthase antibody
      • Glutamine synthetase antibody
      • GS antibody
      • PIG 43 antibody
      • PIG 59 antibody
      • PIG43 antibody
      • PIG59 antibody
      • Proliferation inducing protein 43 antibody
      see all

    References for Anti-Glutamine Synthetase antibody (Biotin) (ab34545)

    ab34545 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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