Anti-Glutamyl hydrolase gamma antibody - C-terminal (ab170729)

Overview

  • Product name
    Anti-Glutamyl hydrolase gamma antibody - C-terminal
    See all Glutamyl hydrolase gamma primary antibodies
  • Description
    Rabbit polyclonal to Glutamyl hydrolase gamma - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Glutamyl hydrolase gamma aa 236-264 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q92820

  • Positive control
    • NCI-H460 cell line lysate; Human liver tissue; NCI-H460 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/10 - 1/50.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 36 kDa.

Target

  • Function
    Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.
  • Sequence similarities
    Belongs to the peptidase C26 family.
    Contains 1 gamma-glutamyl hydrolase domain.
  • Cellular localization
    Secreted > extracellular space. Lysosome. Melanosome. While its intracellular location is primarily the lysosome, most of the enzyme activity is secreted. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Conjugase antibody
    • folylpolygammaglutamyl hydrolase antibody
    • gamma glutamyl hydrolase antibody
    • Gamma-Glu-X carboxypeptidase antibody
    • Gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) antibody
    • Gamma-glutamyl hydrolase antibody
    • GGH antibody
    • GGH_HUMAN antibody
    • gH antibody
    see all

Images

  • Flow cytometric analysis of NCI-H460 cells labeling Glutamyl hydrolase gamma with ab170729 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used.

  • Immunofluorescent analysis of NCI-H460 cells labeling Glutamyl hydrolase gamma with ab170729 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling Glutamyl hydrolase gamma with ab170729 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-Glutamyl hydrolase gamma antibody - C-terminal (ab170729) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

    Predicted band size: 36 kDa

References

ab170729 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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