Anti-Glutamyl hydrolase gamma antibody - N-terminal (ab170315)

Overview

  • Product name
    Anti-Glutamyl hydrolase gamma antibody - N-terminal
    See all Glutamyl hydrolase gamma primary antibodies
  • Description
    Rabbit polyclonal to Glutamyl hydrolase gamma - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Glutamyl hydrolase gamma aa 14-42 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q92820

  • Positive control
    • Human liver tissue; HL-60 cells and lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab170315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 36 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.
  • Sequence similarities
    Belongs to the peptidase C26 family.
    Contains 1 gamma-glutamyl hydrolase domain.
  • Cellular localization
    Secreted > extracellular space. Lysosome. Melanosome. While its intracellular location is primarily the lysosome, most of the enzyme activity is secreted. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Conjugase antibody
    • folylpolygammaglutamyl hydrolase antibody
    • gamma glutamyl hydrolase antibody
    • Gamma-Glu-X carboxypeptidase antibody
    • Gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) antibody
    • Gamma-glutamyl hydrolase antibody
    • GGH antibody
    • GGH_HUMAN antibody
    • gH antibody
    see all

Images

  • Anti-Glutamyl hydrolase gamma antibody - N-terminal (ab170315) at 1/100 dilution + HL-60 cell line lysate at 35 µg

    Predicted band size: 36 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling Glutamyl hydrolase gamma with ab170315 at 1/50 dilution, followed by a peroxidase conjugated secondary antibody and DAB staining.

  • Flow cytometric analysis of HL-60 cells labeling Glutamyl hydrolase gamma with ab170315 at 1/10 dilution (right histogram), compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab170315 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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