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Rabbit polyclonal to Glutamyl Prolyl tRNA synthetase
Predicted to work with:
Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan
Synthetic peptide corresponding to a region between amino acids 950-1000 of Human Glutamyl Prolyl tRNA synthetase (NP_004437.2).
293T, HeLa, Jurkat, TCMK1 and NIH3T3 whole cell lysates
Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab157122 is affinity purified using an epitope specific to Glutamyl Prolyl tRNA synthetase immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 171 kDa.
Use at 2-10 µg/mg of lysate.
Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family. Contains 3 WHEP-TRS domains.
The WHEP-TRS domain is involved in RNA binding.
Phosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.
Information by UniProt
Bifunctional aminoacyl tRNA synthetase antibody
Bifunctional aminoacyl-tRNA synthetase antibody
Bifunctional glutamate/proline tRNA ligase antibody
Western blot - Anti-Glutamyl Prolyl tRNA synthetase antibody (ab157122)
All lanes : Anti-Glutamyl Prolyl tRNA synthetase antibody (ab157122) at 0.1 µg/ml Lane 1 : 293T whole cell lysate Lane 2 : HeLa whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : TCMK1 whole cell lysate Lane 5 : NIH 3T3 whole cell lysate Lysates/proteins at 50 µg per lane. Developed using the ECL technique. Predicted band size: 171 kDa Exposure time: 10 seconds
Immunoprecipitation - Anti-Glutamyl Prolyl tRNA synthetase antibody (ab157122)
Detection of Glutamyl Prolyl tRNA synthetase by Western Blot of Immunprecipitate.
ab157122 at 1 µg/ml labeling Glutamyl Prolyl tRNA synthetase in 293T whole cell lysate immunoprecipitated using ab157122 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"