Anti-Glutamyl Prolyl tRNA synthetase antibody (ab157122)

Overview

  • Product name
    Anti-Glutamyl Prolyl tRNA synthetase antibody
    See all Glutamyl Prolyl tRNA synthetase primary antibodies
  • Description
    Rabbit polyclonal to Glutamyl Prolyl tRNA synthetase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 950-1000 of Human Glutamyl Prolyl tRNA synthetase (NP_004437.2).

  • Positive control
    • 293T, HeLa, Jurkat, TCMK1 and NIH3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab157122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 171 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
  • Sequence similarities
    In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
    In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
    Contains 3 WHEP-TRS domains.
  • Domain
    The WHEP-TRS domain is involved in RNA binding.
  • Post-translational
    modifications
    Phosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bifunctional aminoacyl tRNA synthetase antibody
    • Bifunctional aminoacyl-tRNA synthetase antibody
    • Bifunctional glutamate/proline tRNA ligase antibody
    • Cell proliferation-inducing gene 32 protein antibody
    • DKFZp313B047 antibody
    • EARS antibody
    • Eprs antibody
    • GLNS antibody
    • Glu pro tRNA synthetase antibody
    • GLUPRORS antibody
    • GluRS antibody
    • Glutamate tRNA ligase antibody
    • Glutamatyl prolyl tRNA synthetase antibody
    • Glutaminyl tRNA synthetase antibody
    • Glutamyl prolyl tRNA synthetase antibody
    • Glutamyl tRNA synthetase antibody
    • Glutamyl-tRNA synthetase antibody
    • PARS antibody
    • PIG 32 antibody
    • PIG32 antibody
    • Proliferation inducing gene 32 protein antibody
    • Proliferation inducing protein 32 antibody
    • Proline tRNA ligase antibody
    • Proline--tRNA ligase antibody
    • Prolyl tRNA synthetase antibody
    • Prolyl-tRNA synthetase antibody
    • QARS antibody
    • QPRS antibody
    • SYEP_HUMAN antibody
    see all

Images

  • All lanes : Anti-Glutamyl Prolyl tRNA synthetase antibody (ab157122) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 171 kDa


    Exposure time: 10 seconds
  • Detection of Glutamyl Prolyl tRNA synthetase by Western Blot of Immunprecipitate.

    ab157122 at 1 µg/ml labeling Glutamyl Prolyl tRNA synthetase in 293T whole cell lysate immunoprecipitated using ab157122 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

    Detection: Chemiluminescence with exposure time of 10 seconds

References

ab157122 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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