Anti-Glutamyl Prolyl tRNA synthetase antibody (ab31531)

Overview

  • Product name
    Anti-Glutamyl Prolyl tRNA synthetase antibody
    See all Glutamyl Prolyl tRNA synthetase primary antibodies
  • Description
    Rabbit polyclonal to Glutamyl Prolyl tRNA synthetase
  • Specificity
    This antibody reacts specifically with EPRS protein.
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
  • Immunogen

    V573-K889 native human recombinant EPRS.

Properties

Applications

Our Abpromise guarantee covers the use of ab31531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21460219
WB 1/20000. Predicted molecular weight: 163 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
  • Sequence similarities
    In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
    In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
    Contains 3 WHEP-TRS domains.
  • Domain
    The WHEP-TRS domain is involved in RNA binding.
  • Post-translational
    modifications
    Phosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bifunctional aminoacyl tRNA synthetase antibody
    • Bifunctional aminoacyl-tRNA synthetase antibody
    • Bifunctional glutamate/proline tRNA ligase antibody
    • Cell proliferation-inducing gene 32 protein antibody
    • DKFZp313B047 antibody
    • EARS antibody
    • Eprs antibody
    • GLNS antibody
    • Glu pro tRNA synthetase antibody
    • GLUPRORS antibody
    • GluRS antibody
    • Glutamate tRNA ligase antibody
    • Glutamatyl prolyl tRNA synthetase antibody
    • Glutaminyl tRNA synthetase antibody
    • Glutamyl prolyl tRNA synthetase antibody
    • Glutamyl tRNA synthetase antibody
    • Glutamyl-tRNA synthetase antibody
    • PARS antibody
    • PIG 32 antibody
    • PIG32 antibody
    • Proliferation inducing gene 32 protein antibody
    • Proliferation inducing protein 32 antibody
    • Proline tRNA ligase antibody
    • Proline--tRNA ligase antibody
    • Prolyl tRNA synthetase antibody
    • Prolyl-tRNA synthetase antibody
    • QARS antibody
    • QPRS antibody
    • SYEP_HUMAN antibody
    see all

Anti-Glutamyl Prolyl tRNA synthetase antibody images

  • Anti-Glutamyl Prolyl tRNA synthetase antibody (ab31531) at 1/10000 dilution + HEK293 cell line at 30 µg

    Predicted band size : 163 kDa
    Observed band size : 163 kDa
  • Ab31531 staining Glutamyl Prolyl tRNA synthetase in Hela cell line by Immunofluorescence.
  • Anti-Glutamyl Prolyl tRNA synthetase antibody (ab31531) at 1/20000 dilution + whole cell lysate prepared from HEK-293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 163 kDa
    Observed band size : 155 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab31531 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31531, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab31531 staining Glutamyl Prolyl tRNA synthetase in the Human Liver cell line Huh7 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with Triton X-100 0.5% and blocked with 10% Serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 10% Serum) for 1 hour at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-Glutamyl Prolyl tRNA synthetase antibody (ab31531)

This product has been referenced in:
  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »
  • Guantes R  et al. Global variability in gene expression and alternative splicing is modulated by mitochondrial content. Genome Res 25:633-44 (2015). ICC, Other ; Human . Read more (PubMed: 25800673) »

See all 4 Publications for this product

Product Wall

Application
Immunoprecipitation
Sample
Rat Cell lysate - whole cell (Cardiomyoblast H9c2)
Total protein in input
2e+006 cells
Immuno-precipitation step
Protein A/G
Specification
Cardiomyoblast H9c2
Username

Ahmed Rishiq

Verified customer

Submitted Feb 23 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Liver)
Total protein in input
1e+007 cells
Specification
Liver
Immuno-precipitation step
Other - MagnaChip Protein A+G
Username

Abcam user community

Verified customer

Submitted Aug 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Huh7 (Liver))
Loading amount
1000 cells
Specification
Huh7 (Liver)
Gel Running Conditions
Reduced Denaturing (4-12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Silvia Marquez Jurado

Verified customer

Submitted Aug 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Huh7 (Liver))
Specification
Huh7 (Liver)
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-X100 0,5%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (testis)
Specification
testis
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-X100 0,5%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell lysate)
Loading amount
10 µg
Specification
HeLa cell lysate
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
25 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Alfredo Castello

Verified customer

Submitted Mar 26 2010

Thank you for your email and for the database information for this protein, we'll add the information to the datasheet. This antibody was tested in Western blotting using a HEK293 cell lysate and in IF using Hela cells. We have not tested the antibody ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up