Anti-Glutamyl Prolyl tRNA synthetase antibody (ab85447)

Overview

  • Product name
    Anti-Glutamyl Prolyl tRNA synthetase antibody
    See all Glutamyl Prolyl tRNA synthetase primary antibodies
  • Description
    Rabbit polyclonal to Glutamyl Prolyl tRNA synthetase
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 1439-1488 (GKIVQIPFCG EIDCEDWIKK TTARDQDLEP GAPSMGAKSL CIPFKPLCEL) of Human Glutamyl Prolyl tRNA synthetase (NP_004437).

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 171 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
  • Sequence similarities
    In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
    In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
    Contains 3 WHEP-TRS domains.
  • Domain
    The WHEP-TRS domain is involved in RNA binding.
  • Post-translational
    modifications
    Phosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bifunctional aminoacyl tRNA synthetase antibody
    • Bifunctional aminoacyl-tRNA synthetase antibody
    • Bifunctional glutamate/proline tRNA ligase antibody
    • Cell proliferation-inducing gene 32 protein antibody
    • DKFZp313B047 antibody
    • EARS antibody
    • Eprs antibody
    • GLNS antibody
    • Glu pro tRNA synthetase antibody
    • GLUPRORS antibody
    • GluRS antibody
    • Glutamate tRNA ligase antibody
    • Glutamatyl prolyl tRNA synthetase antibody
    • Glutaminyl tRNA synthetase antibody
    • Glutamyl prolyl tRNA synthetase antibody
    • Glutamyl tRNA synthetase antibody
    • Glutamyl-tRNA synthetase antibody
    • PARS antibody
    • PIG 32 antibody
    • PIG32 antibody
    • Proliferation inducing gene 32 protein antibody
    • Proliferation inducing protein 32 antibody
    • Proline tRNA ligase antibody
    • Proline--tRNA ligase antibody
    • Prolyl tRNA synthetase antibody
    • Prolyl-tRNA synthetase antibody
    • QARS antibody
    • QPRS antibody
    • SYEP_HUMAN antibody
    see all

Images

  • Anti-Glutamyl Prolyl tRNA synthetase antibody (ab85447) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 171 kDa
    Observed band size : 171 kDa

References

ab85447 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab85447.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up