Anti-Glutaredoxin 1 antibody (ab55059)

Overview

  • Product nameAnti-Glutaredoxin 1 antibody
    See all Glutaredoxin 1 primary antibodies
  • Description
    Mouse monoclonal to Glutaredoxin 1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-107 of Human Glutaredoxin 1

Properties

Applications

Our Abpromise guarantee covers the use of ab55059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 12 kDa.
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionHas a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
  • Sequence similaritiesBelongs to the glutaredoxin family.
    Contains 1 glutaredoxin domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • GLRX antibody
    • Glrx1 antibody
    • GLRX1_HUMAN antibody
    • Glutaredoxin antibody
    • Glutaredoxin-1 antibody
    • Glutaredoxin1 antibody
    • Grx 1 antibody
    • Grx antibody
    • Grx1 antibody
    • MGC117407 antibody
    • Thioltransferase 1 antibody
    • Thioltransferase antibody
    • Thioltransferase-1 antibody
    • Thioltransferase1 antibody
    • TTase antibody
    • TTase 1 antibody
    • TTase-1 antibody
    • TTase1 antibody
    see all

Anti-Glutaredoxin 1 antibody images

  • Glutaredoxin 1 antibody (ab55059) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 12 kDa
    Glutaredoxin 1 antibody (ab55059) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
  • Flow Cytometry analysis of Glutaredoxin 1 using ab55059.
    Human monocytes were fixed in paraformaldehyde and permeabilized. ab55059 was used at a 1/250 dilution. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) polyclonal, used at a 1/500 dilution.

    See Abreview

  • ab55059 staining Glutaredoxin 1 in human monocytes by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed, permeabilized, blocked with 2% BSA for 1 hour at 25°C and then incubated with ab55059 at a 1/200 dilution for 4 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L), used at a 1/1000 dilution.

    See Abreview

References for Anti-Glutaredoxin 1 antibody (ab55059)

ab55059 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (RAW 264.7)
Specification RAW 264.7
Treatment Not Treated, Treated with 3.125,12.5 and 50 ug/mL CoO 60nm particles and 250 uM Diamide
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Vamsi Kodali

Verified customer

Submitted Nov 27 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (RAW 264.7)
Specification RAW 264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted May 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Monocytes)
Specification Monocytes
Fixative BD Cytofix/Cytoperm 20 min
Permeabilization Yes - BD Cytofix/Cytoperm 20 min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Monocytes from Apheresis)
Specification Monocytes from Apheresis
Fixation BD Cytofix/Cytoperm 20 min
Permeabilization Yes - BD Cytofix/Cytoperm 20 min
Gating Strategy Monocytes
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"