Overview

  • Product nameAnti-Glutaredoxin 5 antibody
  • Description
    Rabbit polyclonal to Glutaredoxin 5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 72-121 (AVVQILRLHG VRDYAAYNVL DDPELRQGIK DYSNWPTIPQ VYLNGEFVGG) of Human Glutaredoxin 5 (NP_057501).

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab86411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMonothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Required for normal iron homeostasis. Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1.
  • Involvement in diseaseDefects in GLRX5 are a cause of anemia sideroblastic pyridoxine-refractory autosomal recessive (PRARSA) [MIM:205950]. A form of sideroblastic anemia not responsive to pyridoxine. Sideroblastic anemia is characterized by anemia of varying severity, hypochromic peripheral erythrocytes, systemic iron overload secondary to chronic ineffective erythropoiesis, and the presence of bone marrow ringed sideroblasts. Sideroblasts are characterized by iron-loaded mitochondria clustered around the nucleus.
  • Sequence similaritiesBelongs to the glutaredoxin family. Monothiol subfamily.
    Contains 1 glutaredoxin domain.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf87 antibody
    • Chromosome 14 open reading frame 87 antibody
    • FLB4739 antibody
    • GLRX 5 antibody
    • Glrx5 antibody
    • GLRX5_HUMAN antibody
    • Glutaredoxin 5 homolog antibody
    • Glutaredoxin related protein 5 antibody
    • Glutaredoxin-related protein 5 antibody
    • Glutaredoxin5 antibody
    • GRX5 antibody
    • MGC14129 antibody
    • mitochondrial antibody
    • Monothiol glutaredoxin-5 antibody
    • PRO1238 antibody
    see all

Anti-Glutaredoxin 5 antibody images

  • Anti-Glutaredoxin 5 antibody (ab86411) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 17 kDa

References for Anti-Glutaredoxin 5 antibody (ab86411)

This product has been referenced in:
  • Baker PR  et al. Variant non ketotic hyperglycinemia is caused by mutations in LIAS, BOLA3 and the novel gene GLRX5. Brain 137:366-79 (2014). WB ; Human . Read more (PubMed: 24334290) »

See 1 Publication for this product

Product Wall

Following up my message from yesterday, I at least confirmed with our source for this antibody that the immunogen is unique to GLRX5:

"Based on the BLAST results using the sequence of the immunizing peptide, it is uniquely 100% homologous to...

Read More

Your colleague and I discussed some issues your lab was having with Abcam's Glutaredoxin 5 antibody, ab86411. I am waiting for the lab to send any additional testing we have, which I will forward.

Given the scarcity of Glutaredoxin and Bola3...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"