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Products:Tags & Cell Markers >> Fusion / Marker Proteins >> GST
Overview
Product name
Anti-glutathione S transferase Omega 1 antibody
See all glutathione S transferase Omega 1 products (3) ...
Description
Mouse monoclonal to glutathione S transferase Omega 1
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: SKVPSLVGSF IRSQNKEDYA GLKEEFRKEF TKLEEVLTNK KTTFFGGNSI SMIDYLIWPW FERLEAMKLN ECVDHTPKLK LWMAAMKED, corresponding to amino acids 121-210 of Human glutathione S transferase Omega 1
SKVPSLVGSF IRSQNKEDYA GLKEEFRKEF TKLEEVLTNK KTTFFGGNSI SMIDYLIWPW FERLEAMKLN ECVDHTPKLK LWMAAMKED
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 - 5 µg/ml.(This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein)
Target
Relevance
Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities.
Ubiquitous, with highest expression in liver, skeletal muscle and heart and lowest expression
in brain, placenta and lung.
Cellular localization
Cytoplasmic
Alternative names
- AA407097 antibody
- AI194287 antibody
- AU018802 antibody
see all
Database links
- Entrez Gene: 9446 Human
- Omim: 605482 Human
- SwissProt: P78417 Human
- Unigene: 190028 Human
Anti-glutathione S transferase Omega 1 antibody images:
Western blot - glutathione S transferase Omega 1 antibody (ab56550)
Western blot against tagged recombinant protein immunogen using ab56550 glutathione S transferase Omega 1 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
Immunocytochemistry/ Immunofluorescence-glutathione S transferase Omega 1 antibody(ab56550)
ICC/IF image of ab56550 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56550, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-glutathione S transferase Omega 1 antibody (ab56550)
ab56550 has not yet been referenced specifically in any publications.
Publishing research using ab56550? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"