Anti-Glutathione Peroxidase 1 antibody (ab21966)

Overview

  • Product nameAnti-Glutathione Peroxidase 1 antibody
    See all Glutathione Peroxidase 1 primary antibodies
  • Description
    Sheep polyclonal to Glutathione Peroxidase 1
  • Tested applicationsSuitable for: Dot blot, IHC-P, IHC-Fr, WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    RRYSRRFQTIDIEPDIEALL

    , corresponding to C terminal amino acids 175-194 of Human Glutathione Peroxidase

  • General notes


    ab21966 reacts with cellular glutathione peroxidase. This is a 201 amino acid selenium dependant cytoplasmic enzyme that exists as a homotetramer. This enzyme protects red blood cells and other cells from oxidative damage.

Properties

Applications

Our Abpromise guarantee covers the use of ab21966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/500000.

1/500,000 on 500 ng of antigen.

IHC-P 1/1000 - 1/20000.

Dilution depending on tissue and detection method. Antigen retrieval has been used in characterisation but may not be necessary.

IHC-Fr 1/1000 - 1/20000.

Dilution depending on tissue and detection method.

WB 1/10000 - 1/100000. Predicted molecular weight: 22 kDa.

Dilution depending on detection method.

ELISA 1/2000 - 1/20000.

Target

Anti-Glutathione Peroxidase 1 antibody images

  • DAB staining of microglia with GPx1S in human brain tissue. Primary antibody (1/400) secondary antibody donkey anti-sheep - biotin (1/3000) followed by Vector ABC kit.
  • DAB staining of microglia with GPx1S in human brain tissue. Primary antibody (1/1000) secondary antibody donkey anti-sheep - biotin (1/3000) followed by Vector ABC kit. (Oil emersion).

References for Anti-Glutathione Peroxidase 1 antibody (ab21966)

This product has been referenced in:
  • Seeher S  et al. Secisbp2 is essential for embryonic development and enhances selenoprotein expression. Antioxid Redox Signal 21:835-49 (2014). WB ; Mouse . Read more (PubMed: 24274065) »
  • Bansal A  et al. Proteomic analysis reveals late exercise effects on cardiac remodeling following myocardial infarction. J Proteomics 73:2041-9 (2010). WB ; Rat . Read more (PubMed: 20601275) »

See all 4 Publications for this product

Product Wall

Please check the online datasheets of all antibodies. You will find the required information.

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The possible rabbit specific antibodies are available as;


ICAM1: the rabbit protein sequence (http://www.uniprot.org/uniprot/Q75T02) does not show any homology with human protein (http:...

Read More
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Treatment Selenium poor or adequate feeding
Gel Running Conditions Reduced Denaturing (Gel 12.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Aug 26 2009

Thank you for your enquiry and your patience. I apologize for the delay in obtaining the images, however, we now have some excellent images that we added to the online datasheet. This is a high titer antibody and when characterized on dot blots...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"