Anti-Glutathione Peroxidase 1 antibody (ab50427)

Overview

  • Product nameAnti-Glutathione Peroxidase 1 antibody
    See all Glutathione Peroxidase 1 primary antibodies
  • Description
    Goat polyclonal to Glutathione Peroxidase 1
  • SpecificityThis antibody is expected to recognise isoform 1 ( NP_000572.2) only.
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Guinea pig, Chimpanzee, Macaque Monkey, Marmoset (common)
  • Immunogen

    Synthetic peptide:

    C-REALPAPSDDATA

    , corresponding to internal sequence amino acids 128-140 of Human Glutathione Peroxidase 1

  • Positive control
    • Human Liver and kidney lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab50427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 3 - 5 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA Use at an assay dependent concentration. Antibody detection limit dilution 1:32,000.

Target

Anti-Glutathione Peroxidase 1 antibody images

  • ab50427 (20µg/ml) staining of PFA-perfused cryosection of Porcin Kidney following microwave antigen retrieval with citrate buffer pH 3 and CY3-staining shows staining of cytoplasm in PCT epithelial cells.

  • Anti-Glutathione Peroxidase 1 antibody (ab50427) at 1 µg/ml + Human Liver lysate (35µg protein in RIPA buffer).

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab50437 at 3µg/ml staining Glutathione Perxidase 1 in human cerebral cortex by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used for staining.

References for Anti-Glutathione Peroxidase 1 antibody (ab50427)

ab50427 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"