Validated using a knockout cell line
Recombinant
RabMAb

Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)

Overview

  • Product name
    Anti-Glutathione Peroxidase 1 antibody [EPR3311]
    See all Glutathione Peroxidase 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3311] to Glutathione Peroxidase 1
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide. within Human Glutathione Peroxidase 1 aa 1-100 (internal sequence). The exact sequence is proprietary.
    Database link: P07203

  • Positive control
    • Human fetal liver, SH SY5Y, and THP1 cell lysates; Human breast carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.

Target

Anti-Glutathione Peroxidase 1 antibody [EPR3311] images



  • Predicted band size : 22 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)
    Lane 3: THP1 cell lysate (20 µg)
    Lane 4: HL60 cell lysate (20 µg)
    Lanes 1 and 2: Merged signal (red and green). Green - ab108429, observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab108429 was shown to specifically react with Glutathione Peroxidase 1 when Glutathione Peroxidase 1 knockout samples were used. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab108429 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Flow Cytometry analysis of THP-1 (human acute monocytic leukemia ) cells labeling Glutathione Peroxidase 1 with purified ab108429 at 1:250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : SH SY5Y cell lysate
    Lane 3 : THP1 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 22 kDa
  • ab108429, at 1/100 dilution, staining Glutathione Peroxidase 1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

References for Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429)

This product has been referenced in:
  • Chaisiriwong L  et al. A Case-Control Study of Involvement of Oxidative DNA Damage and Alteration of Antioxidant Defense System in Patients with Basal Cell Carcinoma: Modulation by Tumor Removal. Oxid Med Cell Longev 2016:5934024 (2016). IHC . Read more (PubMed: 27057281) »
  • Baqader NO  et al. Nuclear cytoplasmic trafficking of proteins is a major response of human fibroblasts to oxidative stress. J Proteome Res 13:4398-423 (2014). WB ; Human . Read more (PubMed: 25133973) »

See all 2 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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