Anti-Glutathione Peroxidase 2 antibody (ab96798)

Overview

  • Product nameAnti-Glutathione Peroxidase 2 antibody
    See all Glutathione Peroxidase 2 primary antibodies
  • Description
    Rabbit polyclonal to Glutathione Peroxidase 2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1 and 24 of Glutathione Peroxidase 2.

  • Positive control
    • MOLT4 cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: CACO-2.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/1000. Predicted molecular weight: 21 kDa.

Target

  • FunctionCould play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors.
  • Tissue specificityMostly in liver and gastrointestinal tract, not found in heart or kidney.
  • Sequence similaritiesBelongs to the glutathione peroxidase family.
  • Cellular localizationCytoplasm. Mainly cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gastrointestinal glutathione peroxidase antibody
    • GI GPx antibody
    • Glutathione peroxidase 2 (gastrointestinal) antibody
    • Glutathione peroxidase 2 antibody
    • Glutathione peroxidase gastrointestinal antibody
    • Glutathione peroxidase related protein 2 antibody
    • Glutathione peroxidase-gastrointestinal antibody
    • Glutathione peroxidase-related protein 2 antibody
    • GPRP antibody
    • GPRP-2 antibody
    • GPx 2 antibody
    • GPx-2 antibody
    • GPx-GI antibody
    • GPX2 antibody
    • GPX2_HUMAN antibody
    • GSHPx 2 antibody
    • GSHPx GI antibody
    • GSHPx-2 antibody
    • GSHPx-GI antibody
    see all

Anti-Glutathione Peroxidase 2 antibody images

  • ICC/IF image of ab96798 stained CACO-2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96798 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Glutathione Peroxidase 2 antibody (ab96798) at 1/100 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 21 kDa
    15% SDS PAGE

References for Anti-Glutathione Peroxidase 2 antibody (ab96798)

ab96798 has not yet been referenced specifically in any publications.

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