Overview

  • Product nameAnti-Glutathione Peroxidase 3 antibody [23B1]
    See all Glutathione Peroxidase 3 primary antibodies
  • Description
    Mouse monoclonal [23B1] to Glutathione Peroxidase 3
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    His-tagged recombinant human Glutathione peroxidase 3 protein purified from E. coli.

  • Positive control
    • Bosc23 cell lysate transfected with myc-Glutathione Peroxidase 3.

Properties

Applications

Our Abpromise guarantee covers the use of ab27325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500. Predicted molecular weight: 25 kDa.

Target

  • FunctionProtects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.
  • Tissue specificitySecreted in plasma.
  • Sequence similaritiesBelongs to the glutathione peroxidase family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Extracellular glutathione peroxidase antibody
    • Glutathione peroxidase 3 (plasma) antibody
    • Glutathione peroxidase 3 antibody
    • GPX 3 antibody
    • GPx P antibody
    • GPx-3 antibody
    • GPx-P antibody
    • GPX3 antibody
    • GPX3_HUMAN antibody
    • GPXP antibody
    • GSHPx 3 antibody
    • GSHPx P antibody
    • GSHPx-3 antibody
    • GSHPx-P antibody
    • Plasma glutathione peroxidase 3 antibody
    • Plasma glutathione peroxidase antibody
    see all

Anti-Glutathione Peroxidase 3 antibody [23B1] images

  • All lanes : Anti-Glutathione Peroxidase 3 antibody [23B1] (ab27325)

    Lane 1 : Bosc23 cell lysates
    Lane 2 : Bosc23 cell lysate transfected with myc-Glutathione Peroxidase 3


    Predicted band size : 25 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab27325 (4µg/ml) staining Glutathione Peroxidase 3 in human renal cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staininig of renal tubules.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab27325 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27325, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Glutathione Peroxidase 3 antibody [23B1] (ab27325)

This product has been referenced in:
  • Chang SN  et al. Glutathione Peroxidase 3 Inhibits Prostate Tumorigenesis in TRAMP Mice. Prostate 76:1387-98 (2016). Read more (PubMed: 27325372) »
  • Chang SN  et al. High animal fat intake enhances prostate cancer progression and reduces glutathione peroxidase 3 expression in early stages of TRAMP mice. Prostate 74:1266-77 (2014). WB, IHC-P ; Human . Read more (PubMed: 25053105) »

See all 7 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"