Anti-Glutathione Peroxidase 4 antibody [7A4] (ab16737)


  • Product nameAnti-Glutathione Peroxidase 4 antibody [7A4]
    See all Glutathione Peroxidase 4 primary antibodies
  • Description
    Mouse monoclonal [7A4] to Glutathione Peroxidase 4
  • Tested applicationsSuitable for: ELISA, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein purified from E.coli (Human).



Our Abpromise guarantee covers the use of ab16737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at an assay dependent dilution.
    IP: Use at a concentration of 1 - 2 µg/ml.
    WB: 1/2000. Predicted molecular weight: 22 kDa. NOTE: Only recombinant protein is detected by the antibody when Western blotting.
    ICC/IF: Use at a concentration of 1 - 5 µg/ml.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProtects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
    • Tissue specificityPresent primarily in testis.
    • Sequence similaritiesBelongs to the glutathione peroxidase family.
    • Cellular localizationMitochondrion. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Glutathione peroxidase 4 antibody
      • GPX 4 antibody
      • GPX-4 antibody
      • GPX4 antibody
      • GPX4_HUMAN antibody
      • GSHPx-4 antibody
      • MCSP antibody
      • mitochondrial antibody
      • PHGPx antibody
      • Phospholipid hydroperoxidase antibody
      • Phospholipid hydroperoxide glutathione peroxidase antibody
      • Phospholipid hydroperoxide glutathione peroxidase mitochondrial antibody
      • snGPx antibody
      • snPHGPx antibody
      • Sperm nucleus glutathione peroxidase antibody
      see all

    Anti-Glutathione Peroxidase 4 antibody [7A4] images

    • ICC/IF image of ab16737 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16737, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Glutathione Peroxidase 4 antibody [7A4] (ab16737)

    ab16737 has not yet been referenced specifically in any publications.

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    I am sorry these products did not perform as stated on the datasheets and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number *****. To check the status of the order please contact our C...

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    Thank you for your enquiry and patience. I contacted the originator of ab16737 and they informed me that only recombinant protein is detected by this antibody when Western blotting. Thus, we think this is the reason that you are not seeing a signal wit...

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