Anti-Glutathione Peroxidase 4 antibody (ab231174)
Key features and details
- Rabbit polyclonal to Glutathione Peroxidase 4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Glutathione Peroxidase 4 antibody
See all Glutathione Peroxidase 4 primary antibodies -
Description
Rabbit polyclonal to Glutathione Peroxidase 4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Orangutan -
Immunogen
Recombinant fragment corresponding to Human Glutathione Peroxidase 4 aa 50 to the C-terminus. (N-terminal His-tag and GST-tag; Expressed in E.coli).
Database link: P36969 -
Positive control
- IHC-P: Human breast cancer, thyroid cancer, liver, liver cancer and kidney tissues. WB: Human liver lysate; HEK-293T cell lysate; Mouse testis lysate; Recombinant human Glutathione Peroxidase 4 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231174 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 22 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 22 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. -
Tissue specificity
Present primarily in testis. -
Sequence similarities
Belongs to the glutathione peroxidase family. -
Cellular localization
Mitochondrion. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 286809 Cow
- Entrez Gene: 2879 Human
- Entrez Gene: 625249 Mouse
- Entrez Gene: 29328 Rat
- Omim: 138322 Human
- SwissProt: Q9N2J2 Cow
- SwissProt: P36969 Human
- SwissProt: O70325 Mouse
see all -
Alternative names
- Glutathione peroxidase 4 antibody
- GPX 4 antibody
- GPX-4 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human kidney tissue stained for Glutathione Peroxidase 4 using ab231174 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human breast cancer tissue stained for Glutathione Peroxidase 4 using ab231174 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human thyroid cancer tissue stained for Glutathione Peroxidase 4 using ab231174 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human liver tissue stained for Glutathione Peroxidase 4 using ab231174 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human liver cancer tissue stained for Glutathione Peroxidase 4 using ab231174 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human kidney tissue stained for Glutathione Peroxidase 4 using ab231174 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-Glutathione Peroxidase 4 antibody (ab231174) at 2 µg/ml + Recombinant human Glutathione Peroxidase 4 protein
Predicted band size: 22 kDa -
Anti-Glutathione Peroxidase 4 antibody (ab231174) at 2 µg/ml + Human liver lysate
Predicted band size: 22 kDa -
All lanes : Anti-Glutathione Peroxidase 4 antibody (ab231174) at 2 µg/ml
Lane 1 : Wild-type HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Glutathione Peroxidase 4 knockout HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Predicted band size: 22 kDa -
Anti-Glutathione Peroxidase 4 antibody (ab231174) at 2 µg/ml + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Predicted band size: 22 kDa -
Anti-Glutathione Peroxidase 4 antibody (ab231174) at 2 µg/ml + Mouse testis lysate
Predicted band size: 22 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab231174 has been referenced in 5 publications.
- Bin S et al. Targeting miR-10a-5p/IL-6R axis for reducing IL-6-induced cartilage cell ferroptosis. Exp Mol Pathol 118:104570 (2021). PubMed: 33166496
- Wu P et al. Circular RNA circEPSTI1 accelerates cervical cancer progression via miR-375/409-3P/515-5p-SLC7A11 axis. Aging (Albany NY) 13:4663-4673 (2021). PubMed: 33534779
- Zhao Y et al. iTRAQ-based proteomic analysis of sperm reveals candidate proteins that affect the quality of spermatozoa from boars on plateaus. Proteome Sci 19:9 (2021). PubMed: 34330296
- Chen W et al. Exosomal MIF Derived From Nasopharyngeal Carcinoma Promotes Metastasis by Repressing Ferroptosis of Macrophages. Front Cell Dev Biol 9:791187 (2021). PubMed: 35036405
- Zhang H et al. miR-30-5p-mediated ferroptosis of trophoblasts is implicated in the pathogenesis of preeclampsia. Redox Biol 29:101402 (2020). PubMed: 31926626