The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
FunctionProtects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
Tissue specificityPresent primarily in testis.
Sequence similaritiesBelongs to the glutathione peroxidase family.
Western blot - Glutathione Peroxidase 4 antibody (ab40993)
All lanes : Anti-Glutathione Peroxidase 4 antibody (ab40993) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Human testis tissue lysate - total protein (ab30257) Lane 3 : Human liver tissue lysate - total protein (ab29889) Lane 4 : Human heart tissue lysate - total protein (ab29431) Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330) Lane 6 : Human kidney tissue lysate - total protein (ab30203) Lane 7 : Human colon tissue lysate - total protein (ab30051)
ICC/IF image of ab40993 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40993, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-Glutathione Peroxidase 4 antibody (ab40993)
This product has been referenced in:
Hauser DN et al. Dopamine quinone modifies and decreases the abundance of the mitochondrial selenoprotein glutathione peroxidase 4. Free Radic Biol Med65:419-27 (2013).
Read more (PubMed: 23816523) »