The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 22 kDa).
Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
ab51944 at 0.3ug/ml staining Glutathione peroxidase 4 in human testis tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave with citrate buffer (pH 6.0). The HRP-staining procedure was used for detection.