Anti-Glutathione Peroxidase 4 antibody (ab51944)

Overview

  • Product nameAnti-Glutathione Peroxidase 4 antibody
    See all Glutathione Peroxidase 4 primary antibodies
  • Description
    Goat polyclonal to Glutathione Peroxidase 4
  • Specificityab51944 is expected to recognise isoform A and isoform C.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide:

    CEEPLVIEKDLPHY

    , corresponding to C terminal amino acids 184-196 of Glutathione Peroxidase 4

  • Positive control
    • Human Kidney and Testis lysates and HEK293 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab51944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 22 kDa).

Target

  • FunctionProtects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
  • Tissue specificityPresent primarily in testis.
  • Sequence similaritiesBelongs to the glutathione peroxidase family.
  • Cellular localizationMitochondrion. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glutathione peroxidase 4 antibody
    • GPX 4 antibody
    • GPX-4 antibody
    • GPX4 antibody
    • GPX4_HUMAN antibody
    • GSHPx-4 antibody
    • MCSP antibody
    • mitochondrial antibody
    • PHGPx antibody
    • Phospholipid hydroperoxidase antibody
    • Phospholipid hydroperoxide glutathione peroxidase antibody
    • Phospholipid hydroperoxide glutathione peroxidase mitochondrial antibody
    • snGPx antibody
    • snPHGPx antibody
    • Sperm nucleus glutathione peroxidase antibody
    see all

Anti-Glutathione Peroxidase 4 antibody images

  • Anti-Glutathione Peroxidase 4 antibody (ab51944) at 0.1 µg/ml + 35 µg of Human Testis lysate (in RIPA buffer)

    Predicted band size : 22 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
  • ab51944 at 0.3ug/ml staining Glutathione peroxidase 4 in human testis tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave with citrate buffer (pH 6.0). The HRP-staining procedure was used for detection.

References for Anti-Glutathione Peroxidase 4 antibody (ab51944)

ab51944 has not yet been referenced specifically in any publications.

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