Anti-Glutathione Peroxidase 7 antibody (ab51948)

Overview

  • Product nameAnti-Glutathione Peroxidase 7 antibody
    See all Glutathione Peroxidase 7 primary antibodies
  • Description
    Goat polyclonal to Glutathione Peroxidase 7
  • Tested applicationsSuitable for: Sandwich ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    CNQFGQQEPDSNK

    , corresponding to internal sequence amino acids 86-98 of Human Glutathione Peroxidase 7

  • Positive control
    • Human Prostate lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab51948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa). An additional band of unknown identity was also consistently observed at 40kDa. This band was successfully blocked by incubation with the immunising peptide.
ELISA 1/32000.

Target

Anti-Glutathione Peroxidase 7 antibody images

  • All lanes : Anti-Glutathione Peroxidase 7 antibody (ab51948) at 0.3 µg/ml

    Lane 1 : Human Prostate lysate (in RIPA buffer)
    Lane 2 : Human Prostate lysate (in RIPA buffer) with immunising peptide

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 21 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • Sandwich-type ELISA with increasing amount of recombinant GPX7 captured by a rabbit antibody. ab51948 used as reporter at 5ug/ml.

References for Anti-Glutathione Peroxidase 7 antibody (ab51948)

ab51948 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"