Overview

  • Product name
    Anti-Glutathione Peroxidase 7 antibody
    See all Glutathione Peroxidase 7 primary antibodies
  • Description
    Goat polyclonal to Glutathione Peroxidase 7
  • Host species
    Goat
  • Tested applications
    Suitable for: Sandwich ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    CNQFGQQEPDSNK

    , corresponding to internal sequence amino acids 86-98 of Human Glutathione Peroxidase 7

  • Positive control
    • Human Prostate lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab51948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa). An additional band of unknown identity was also consistently observed at 40kDa. This band was successfully blocked by incubation with the immunising peptide.
ELISA 1/32000.

Target

Images

  • All lanes : Anti-Glutathione Peroxidase 7 antibody (ab51948) at 0.3 µg/ml

    Lane 1 : Human Prostate lysate (in RIPA buffer)
    Lane 2 : Human Prostate lysate (in RIPA buffer) with immunising peptide

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 21 kDa
    Observed band size: 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.

  • Sandwich-type ELISA with increasing amount of recombinant GPX7 captured by a rabbit antibody. ab51948 used as reporter at 5ug/ml.

References

This product has been referenced in:
  • Joyce NC  et al. Age-related gene response of human corneal endothelium to oxidative stress and DNA damage. Invest Ophthalmol Vis Sci 52:1641-9 (2011). Read more (PubMed: 21087955) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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