The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa). An additional band of unknown identity was also consistently observed at 40kDa. This band was successfully blocked by incubation with the immunising peptide.