Anti-Glutathione Reductase antibody (ab16801)

Overview

  • Product name
    Anti-Glutathione Reductase antibody
    See all Glutathione Reductase primary antibodies
  • Description
    Rabbit polyclonal to Glutathione Reductase
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein purified from E.coli (Human).

  • Positive control
    • HeLa or Jurkat whole cell lysate. Mouse or Rat lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/400.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000. Predicted molecular weight: 58 kDa.

Target

Images



  • Predicted band size : 58 kDa
    ab16801 at a 1/2000 dilution staining approx. 58kDa Glutathione peroxidase in 1) HeLa cell lysate, 2) Jurkat cell lysate, 3) Mouse lung kysates and 4) Rat lung lysate by Western blot (ECL). ab16801 at a 1/2000 dilution staining approx. 58kDa Glutathione reductase in 1) HeLa cell lysate, 2) Jurkat cell lysate, 3) Mouse lung kysates and 4) Rat lung lysate by Western blot (ECL).
  • ICC/IF image of ab16801 stained MCF7 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16801, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • ab16801 staining Glutathione Reductase in rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in blocking buffer) for 15 hours at 4°C. ab6721 (1/500) was used as the secondary antibody.

    See Abreview

  • Ab6801 staining Human normal placenta. Staining is localized to the cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References

This product has been referenced in:
  • Shanmugam G  et al. Constitutive activation of Nrf2 induces a stable reductive state in the mouse myocardium. Redox Biol 12:937-945 (2017). WB ; Mouse . Read more (PubMed: 28482326) »
  • Sollanek KJ  et al. Global Proteome Changes in the Rat Diaphragm Induced by Endurance Exercise Training. PLoS One 12:e0171007 (2017). Read more (PubMed: 28135290) »

See all 20 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Placenta)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
Placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Polina Vishnyakova

Verified customer

Submitted Jun 23 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate acid solution
Permeabilization
No
Specification
kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Miss. Jin Young Park

Verified customer

Submitted Feb 09 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (kidney)
Antigen retrieval step
None
Permeabilization
No
Specification
kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 15 2016

Application
Western blot
Loading amount
36 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Tissue lysate - whole (Bone tissue (Tibia))
Specification
Bone tissue (Tibia)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Helder Fonseca

Verified customer

Submitted Oct 06 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skeletal muscle)
Specification
Skeletal muscle
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 32°C
Username

Mr. Mtuhtu S.Gounder

Verified customer

Submitted Jan 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Loading amount
30 µg
Specification
Skeletal muscle
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 30°C
Username

Mr. Mtuhtu S.Gounder

Verified customer

Submitted Nov 30 2011

I have processed your request to receive ab106843 as a replacement for ab16801. I have also sent ab97055, goat polyclonal to rat (Biotin) aswell. The ab106843 is backordered until December 12th though and so your order will ship as soon as it comes in...

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Thank you for your reply. I would be happy to send you ab106843 as a replacement. The secondary antibody that you originally ordered was an anti-rabbit, not an anti-mouse, is that correct? I would also be happy to send you ab97055 as well. ...

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Thank you for your reply. The antibody ab55075 has not been proven to work on mouse tissue, there is a chance that the antibody will work on mouse tissue but we cannot guarantee it. The only other antibody in our catalogue that will work on mouse tissu...

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