Anti-Glutathione Reductase antibody (ab84963)

Overview

  • Product nameAnti-Glutathione Reductase antibody
    See all Glutathione Reductase primary antibodies
  • Description
    Rabbit polyclonal to Glutathione Reductase
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, African Green Monkey
  • Immunogen

    Synthetic peptide within residues: PKPTIEVSGK KYTAPHILIA TGGMPSTPHE SQIPGASLGI TSDGFFQLEE , corresponding to internal sequence amino acids 180-229 of Human Glutathione Reductase (NP_000628)

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

Target

Anti-Glutathione Reductase antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling Glutathione Reductase with ab84963 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Glutathione Reductase. Right - Merge.
  • Anti-Glutathione Reductase antibody (ab84963) at 1 µg/ml (5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Gel concentration: 12%

References for Anti-Glutathione Reductase antibody (ab84963)

ab84963 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HCT116 colon cancer cell line)
Loading amount 60 µg
Specification HCT116 colon cancer cell line
Treatment different treatments
Gel Running Conditions Reduced Denaturing (13%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Christian Marx

Verified customer

Submitted Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"