Anti-Glutathione Reductase antibody (ab87330)

Overview

  • Product nameAnti-Glutathione Reductase antibody
    See all Glutathione Reductase primary antibodies
  • Description
    Rabbit polyclonal to Glutathione Reductase
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Marmoset (common)
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Glutathione Reductase.

    (Peptide available as ab95921.)

  • Positive control
    • This antibody gave a positive signal in HeLa and Jurkat whole cell lysates and in the following human tissue lysates: Brain; Liver; Testis; Lung; Heart.

Properties

Applications

Our Abpromise guarantee covers the use of ab87330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 56 kDa).

Target

  • FunctionMaintains high levels of reduced glutathione in the cytosol.
  • Sequence similaritiesBelongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
  • DomainEach subunit can be divided into 4 domains that are consecutive along the polypeptide chain. Domains 1 and 2 bind FAD and NADPH, respectively. Domain 4 forms the interface.
  • Cellular localizationCytoplasm and Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • GLR1 antibody
    • GLUR antibody
    • Glutathione reductase antibody
    • Glutathione reductase mitochondrial antibody
    • Glutathione reductase, mitochondrial antibody
    • GR antibody
    • Gr1 antibody
    • GRase antibody
    • GRD 1 antibody
    • GRD1 antibody
    • GSHR_HUMAN antibody
    • GSR antibody
    • MGC78522 antibody
    see all

Anti-Glutathione Reductase antibody images

  • All lanes : Anti-Glutathione Reductase antibody (ab87330) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : Human liver tissue lysate - total protein (ab29889)
    Lane 5 : Human testis tissue lysate - total protein (ab30257)
    Lane 6 : Lung (Human) Tissue Lysate
    Lane 7 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 37 kDa,50 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds

References for Anti-Glutathione Reductase antibody (ab87330)

ab87330 has not yet been referenced specifically in any publications.

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