Western blot - Glutathione Reductase antibody (ab87330)
All lanes : Anti-Glutathione Reductase antibody (ab87330) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : Human brain tissue lysate - total protein (ab29466) Lane 4 : Human liver tissue lysate - total protein (ab29889) Lane 5 : Human testis tissue lysate - total protein (ab30257) Lane 6 : Lung (Human) Tissue Lysate Lane 7 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa Observed band size: 56 kDa Additional bands at: 37 kDa, 50 kDa, 90 kDa. We are unsure as to the identity of these extra bands.