Validated using a knockout cell line
Recombinant
RabMAb

Anti-Glutathione Reductase antibody [EPR7238] (ab124995)

Overview

  • Product name
    Anti-Glutathione Reductase antibody [EPR7238]
    See all Glutathione Reductase primary antibodies
  • Description
    Rabbit monoclonal [EPR7238] to Glutathione Reductase
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Glutathione Reductase.

  • Positive control
    • Jurkat, HeLa, A549 and Caco-2 cell lysates; permeabilized HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    Images



    • Predicted band size : 56 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Glutathione Reductase knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab124995 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab124995 was shown to specifically react with Glutathione Reductase when Glutathione Reductase knockout samples were used. Wild-type and Glutathione Reductase knockout samples were subjected to SDS-PAGE.  Ab124995 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • ab124995, at a 1/10 dilution, staining Glutathione Reductase in permeabilized HeLa cells by Flow Cytometry (red). Analysis with a rabbit IgG (negative control) is shown in green.
    • All lanes : Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/2000 dilution

      Lane 1 : mouse brain
      Lane 2 : rat brain

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 56 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 56 kDa
    • All lanes : Anti-Glutathione Reductase antibody [EPR7238] (ab124995) at 1/2000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : Mouse kidney tissue lysate
      Lane 4 : Mouse


      Predicted band size : 56 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

    References

    This product has been referenced in:
    • Dalmases M  et al. Brain tissue hypoxia and oxidative stress induced by obstructive apneas is different in young and aged rats. Sleep 37:1249-56 (2014). Read more (PubMed: 25061253) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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