Anti-Glutathione S Transferase alpha 1 antibody (ab53940)

Overview

  • Product name
    Anti-Glutathione S Transferase alpha 1 antibody
    See all Glutathione S Transferase alpha 1 primary antibodies
  • Description
    Goat polyclonal to Glutathione S Transferase alpha 1
  • Specificity
    The antibody is known to be class-specific and recognizes alpha-form GSTs from rat, mouse, hamster, rabbit, and human. Recognizes GST A1-1(Ya1), A2-2 (Ya2,Yc2), A3-3 (Ya3,Yc), A4-4 (Yk).
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Human
  • Immunogen

    Full length native protein (purified) corresponding to Rat Glutathione S Transferase alpha 1.
    Database link: P08263

Properties

Applications

Our Abpromise guarantee covers the use of ab53940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).

5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature.

 

Target

  • Function
    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • Tissue specificity
    Liver.
  • Sequence similarities
    Belongs to the GST superfamily. Alpha family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Domain
    The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glutathione S alkyltransferase A1 antibody
    • Glutathione S aryltransferase A1 antibody
    • Glutathione S transferase 2 antibody
    • Glutathione S transferase A1 antibody
    • Glutathione S transferase alpha 1 antibody
    • Glutathione S transferase Ha subunit 1 antibody
    • Glutathione S-transferase A1 antibody
    • GST class-alpha member 1 antibody
    • GST epsilon antibody
    • GST HA subunit 1 antibody
    • GST, class alpha, 1 antibody
    • GST-epsilon antibody
    • GST2 antibody
    • GSTA1 1 antibody
    • GSTA1 antibody
    • GSTA1-1 antibody
    • GSTA1_HUMAN antibody
    • GTH1 antibody
    • HA subunit 1 antibody
    • MGC131939 antibody
    • OTTHUMP00000016611 antibody
    • S (hydroxyalkyl)glutathione lyase A1 antibody
    see all

Anti-Glutathione S Transferase alpha 1 antibody images

  • A. Negative control (no antibody).

    B. Rat liver section stained with ab53940 at 1/1000 dilution followed by anti-goat IgG-HRP with DAB HRP substrate.

  • ICC/IF image of ab53940 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53940, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.



  • Predicted band size : 26 kDa
    Lane 1. Molecular Weight Marker
    Lane 2. GST α Standard

    A 1:2000 dilution is recommended for ECL method with overnight incubation at room temperature.

References for Anti-Glutathione S Transferase alpha 1 antibody (ab53940)

This product has been referenced in:
  • Singh A  et al. Podophyllotoxin and Rutin Modulates Ionizing Radiation-Induced Oxidative Stress and Apoptotic Cell Death in Mice Bone Marrow and Spleen. Front Immunol 8:183 (2017). Read more (PubMed: 28289414) »
  • Grootaert MO  et al. Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis. Autophagy 11:2014-2032 (2015). WB ; Mouse . Read more (PubMed: 26391655) »

See all 4 Publications for this product

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