Anti-Glutathione S Transferase alpha 1 antibody (ab53940)


  • Product nameAnti-Glutathione S Transferase alpha 1 antibody
    See all Glutathione S Transferase alpha 1 primary antibodies
  • Description
    Goat polyclonal to Glutathione S Transferase alpha 1
  • Specificityab53940 is specific for glutathione S Transferase (GST) alpha.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Human
  • Immunogen

    Full length native protein (purified) corresponding to Rat Glutathione S Transferase alpha 1.



Our Abpromise guarantee covers the use of ab53940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/3000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).


  • FunctionConjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • Tissue specificityLiver.
  • Sequence similaritiesBelongs to the GST superfamily. Alpha family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • DomainThe C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glutathione S alkyltransferase A1 antibody
    • Glutathione S aryltransferase A1 antibody
    • Glutathione S transferase 2 antibody
    • Glutathione S transferase A1 antibody
    • Glutathione S transferase alpha 1 antibody
    • Glutathione S transferase Ha subunit 1 antibody
    • Glutathione S-transferase A1 antibody
    • GST class-alpha member 1 antibody
    • GST epsilon antibody
    • GST HA subunit 1 antibody
    • GST, class alpha, 1 antibody
    • GST-epsilon antibody
    • GST2 antibody
    • GSTA1 1 antibody
    • GSTA1 antibody
    • GSTA1-1 antibody
    • GSTA1_HUMAN antibody
    • GTH1 antibody
    • HA subunit 1 antibody
    • MGC131939 antibody
    • OTTHUMP00000016611 antibody
    • S (hydroxyalkyl)glutathione lyase A1 antibody
    see all

Anti-Glutathione S Transferase alpha 1 antibody images

  • ICC/IF image of ab53940 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53940, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Predicted band size : 26 kDa
    Lane 1. Molecular Weight Marker
    Lane 2. GST α Standard

    A 1:2000 dilution is recommended for ECL method with overnight incubation at room temperature.

References for Anti-Glutathione S Transferase alpha 1 antibody (ab53940)

This product has been referenced in:
  • Volkova M  et al. Activation of the aryl hydrocarbon receptor by doxorubicin mediates cytoprotective effects in the heart. Cardiovasc Res 90:305-14 (2011). WB ; Rat . Read more (PubMed: 21233252) »
  • Azkargorta M  et al. Differential proteomics analysis reveals a role for E2F2 in the regulation of the Ahr pathway in T lymphocytes. Mol Cell Proteomics 9:2184-94 (2010). WB ; Mouse . Read more (PubMed: 20573986) »

See all 2 Publications for this product

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