Anti-Glutathione S Transferase alpha 1 antibody (ab96043)

Overview

  • Product nameAnti-Glutathione S Transferase alpha 1 antibody
    See all Glutathione S Transferase alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to Glutathione S Transferase alpha 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Glutathione S Transferase alpha 1 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab107709)

  • Positive control
    • This antibody gave a positive signal in both Human and Rat Liver tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab96043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • FunctionConjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • Tissue specificityLiver.
  • Sequence similaritiesBelongs to the GST superfamily. Alpha family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • DomainThe C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glutathione S alkyltransferase A1 antibody
    • Glutathione S aryltransferase A1 antibody
    • Glutathione S transferase 2 antibody
    • Glutathione S transferase A1 antibody
    • Glutathione S transferase alpha 1 antibody
    • Glutathione S transferase Ha subunit 1 antibody
    • Glutathione S-transferase A1 antibody
    • GST class-alpha member 1 antibody
    • GST epsilon antibody
    • GST HA subunit 1 antibody
    • GST, class alpha, 1 antibody
    • GST-epsilon antibody
    • GST2 antibody
    • GSTA1 1 antibody
    • GSTA1 antibody
    • GSTA1-1 antibody
    • GSTA1_HUMAN antibody
    • GTH1 antibody
    • HA subunit 1 antibody
    • MGC131939 antibody
    • OTTHUMP00000016611 antibody
    • S (hydroxyalkyl)glutathione lyase A1 antibody
    see all

Anti-Glutathione S Transferase alpha 1 antibody images

  • All lanes : Anti-Glutathione S Transferase alpha 1 antibody (ab96043) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Additional bands at : 45 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa


    Exposure time : 8 minutes

References for Anti-Glutathione S Transferase alpha 1 antibody (ab96043)

ab96043 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Zebrafish Cell (Whole mount Immunofluorescence)
Permeabilization Yes - Methanol
Specification Whole mount Immunofluorescence
Blocking step 2% BSA + 10 % Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Paraformaldehyde
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Submitted Feb 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"