Anti-Glutathione S Transferase alpha 1 antibody (ab96043)

Overview

  • Product name
    Anti-Glutathione S Transferase alpha 1 antibody
    See all Glutathione S Transferase alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to Glutathione S Transferase alpha 1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Glutathione S Transferase alpha 1 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab107709)

  • Positive control
    • This antibody gave a positive signal in both Human and Rat Liver tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab96043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

Target

  • Function
    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • Tissue specificity
    Liver.
  • Sequence similarities
    Belongs to the GST superfamily. Alpha family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Domain
    The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glutathione S alkyltransferase A1 antibody
    • Glutathione S aryltransferase A1 antibody
    • Glutathione S transferase 2 antibody
    • Glutathione S transferase A1 antibody
    • Glutathione S transferase alpha 1 antibody
    • Glutathione S transferase Ha subunit 1 antibody
    • Glutathione S-transferase A1 antibody
    • GST class-alpha member 1 antibody
    • GST epsilon antibody
    • GST HA subunit 1 antibody
    • GST, class alpha, 1 antibody
    • GST-epsilon antibody
    • GST2 antibody
    • GSTA1 1 antibody
    • GSTA1 antibody
    • GSTA1-1 antibody
    • GSTA1_HUMAN antibody
    • GTH1 antibody
    • HA subunit 1 antibody
    • MGC131939 antibody
    • OTTHUMP00000016611 antibody
    • S (hydroxyalkyl)glutathione lyase A1 antibody
    see all

Anti-Glutathione S Transferase alpha 1 antibody images

  • All lanes : Anti-Glutathione S Transferase alpha 1 antibody (ab96043) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Additional bands at : 45 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • All lanes : Anti-Glutathione S Transferase alpha 1 antibody (ab96043) at 1 µg/ml

    Lane 1 : Recombinant Human Glutathione S Transferase alpha 1 protein (ab95381) at 0.1 µg
    Lane 2 : Recombinant Human Glutathione S Transferase alpha 1 protein (ab95381) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa


    Exposure time : 8 minutes

References for Anti-Glutathione S Transferase alpha 1 antibody (ab96043)

This product has been referenced in:
  • Civantos E  et al. Sitagliptin ameliorates oxidative stress in experimental diabetic nephropathy by diminishing the miR-200a/Keap-1/Nrf2 antioxidant pathway. Diabetes Metab Syndr Obes 10:207-222 (2017). WB ; Rat . Read more (PubMed: 28652790) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Zebrafish Cell (Whole mount Immunofluorescence)
Permeabilization
Yes - Methanol
Specification
Whole mount Immunofluorescence
Blocking step
2% BSA + 10 % Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Paraformaldehyde
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Submitted Feb 20 2017

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