Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)

Overview

  • Product nameAnti-Glutathione S Transferase mu antibody [EPR1936(2)]
    See all Glutathione S Transferase mu primary antibodies
  • Description
    Rabbit monoclonal [EPR1936(2)] to Glutathione S Transferase mu
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Glutathione S Transferase mu aa 100-200. The exact sequence is proprietary.
    (Peptide available as ab180487)

  • Positive control
    • WB: HeLa, HepG2, SH-SY5Y and NIH/3T3 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab108524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.Can be blocked with Glutathione S Transferase mu peptide (ab180487).
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • FunctionConjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
    • Tissue specificityLiver (at protein level).
    • Sequence similaritiesBelongs to the GST superfamily. Mu family.
      Contains 1 GST C-terminal domain.
      Contains 1 GST N-terminal domain.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Glutathione S alkyltransferase antibody
      • Glutathione S aralkyltransferase antibody
      • Glutathione S aryltransferase antibody
      • Glutathione S transferase M1 antibody
      • Glutathione S transferase mu 1 antibody
      • Glutathione S transferase mu antibody
      • Glutathione S-transferase Mu 1 antibody
      • GST class mu 1 antibody
      • GST class Mu antibody
      • GST class-mu 1 antibody
      • GST HB subunit 4 antibody
      • GST mu antibody
      • GST1 antibody
      • GSTM1 1 antibody
      • Gstm1 antibody
      • GSTM1-1 antibody
      • GSTM1_HUMAN antibody
      • GSTM1a 1a antibody
      • GSTM1a-1a antibody
      • GSTM1b 1b antibody
      • GSTM1b-1b antibody
      • GTH4 antibody
      • GTM1 antibody
      • H B antibody
      • HB subunit 4 antibody
      • MGC26563 antibody
      • MU 1 antibody
      • MU antibody
      • S (hydroxyalkyl)glutathione lyase antibody
      see all

    Anti-Glutathione S Transferase mu antibody [EPR1936(2)] images

    • All lanes : Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution (purified)

      Lane 1 : HeLa whole cell lysate
      Lane 2 : SH-SY5Y whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 26 kDa
      Observed band size : 26 kDa
      Blocking and dilution buffer: 5% NFDM/TBST
    • Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution (purified) + NIH/3T3 whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 26 kDa
      Observed band size : 26 kDa
      Blocking and dilution buffer: 5% NFDM/TBST
    • Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution (purified) + PC-12 whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 26 kDa
      Observed band size : 26 kDa


      Exposure time : 30 seconds

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 26 kDa

    References for Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524)

    ab108524 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions Reduced Denaturing (15%)
    Loading amount 20 µg
    Specification Skeletal muscle
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 18 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions Reduced Denaturing (15%)
    Loading amount 20 µg
    Specification Skeletal muscle
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 18 2016

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (15% Gel)
    Sample Mouse Tissue lysate - whole (Skeletal muscle)
    Specification Skeletal muscle
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 04 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"