Anti-glutathione S transferase Omega 1 antibody (ab174253)

Overview

  • Product name
    Anti-glutathione S transferase Omega 1 antibody
    See all glutathione S transferase Omega 1 primary antibodies
  • Description
    Rabbit polyclonal to glutathione S transferase Omega 1
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human glutathione S transferase Omega 1 aa 133-163 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P78417

  • Positive control
    • NCI-H460 cell line lysates; NCI-H292 cells; Human skeletal muscle tissue; 293 cells transiently transfected with the glutathione S transferase Omega 1 gene.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab174253 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/50 - 1/100. Predicted molecular weight: 28 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.
  • Tissue specificity
    Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart. Lowest expression in brain, placenta and lung.
  • Sequence similarities
    Belongs to the GST superfamily. Omega family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Cellular localization
    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA407097 antibody
    • AI194287 antibody
    • AU018802 antibody
    • DKFZp686H13163 antibody
    • EC 2.5.1.18 antibody
    • Glutathione S transferase omega 1 antibody
    • Glutathione S-transferase omega 1-1 antibody
    • Glutathione S-transferase omega antibody
    • Glutathione S-transferase omega-1 antibody
    • Glutathione transferase omega 1 antibody
    • Glutathione-dependent dehydroascorbate reductase antibody
    • Glutathione-S-transferase like antibody
    • Glutathione-S-transferase omega 1 antibody
    • GSTO 1-1 antibody
    • GSTO-1 antibody
    • GSTO1 1 antibody
    • Gsto1 antibody
    • GSTO1_HUMAN antibody
    • GSTTLp28 antibody
    • GSTX antibody
    • Gtsttl antibody
    • MGC94845 antibody
    • MMA(V) reductase antibody
    • Monomethylarsonic acid reductase antibody
    • OTTHUMP00000020429 antibody
    • p28 antibody
    • S-(Phenacyl)glutathione reductase antibody
    • SPG-R antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skeletal muscle tissue labeling glutathione S transferase Omega 1 with ab174253 at 1/50 dilution. Detection utilised peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-glutathione S transferase Omega 1 antibody (ab174253) at 1/50 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 28 kDa
  • All lanes : Anti-glutathione S transferase Omega 1 antibody (ab174253) at 1/50 dilution

    Lane 1 : Non-transfected 293 cell lysates
    Lane 2 : 293 cell lysates transiently transfected with the glutathione S transferase Omega 1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 28 kDa
  • Flow cytometric analysis of NCI-H292 cells labeling glutathione S transferase Omega 1 with ab174253 at 1/10 (lower histogram) compared to a negative control cell (upper histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.

References

ab174253 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
ELISA
Sample
Human Recombinant protein (Expression system: Escherichia coli)
Specification
Expression system: Escherichia coli
Blocking step
PBS / Tween 20 as blocking agent for 40 minute(s) · Concentration: 3% · Temperature: RT°C
Type
Indirect
Username

Luigina De Leo

Verified customer

Submitted Dec 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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