Anti-Glutathione Synthetase antibody (ab91591)

Overview

  • Product name
    Anti-Glutathione Synthetase antibody
    See all Glutathione Synthetase primary antibodies
  • Description
    Rabbit polyclonal to Glutathione Synthetase
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C terminal region of Human Glutathione Synthetase.

  • Positive control
    • Mouse liver tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab91591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 52 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA 1/1000.

Target

  • Pathway
    Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.
  • Involvement in disease
    Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) [MIM:266130]; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
    Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia.
  • Sequence similarities
    Belongs to the eukaryotic GSH synthase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • epididymis secretory sperm binding protein Li 64p antibody
    • epididymis secretory sperm binding protein Li 88n antibody
    • Glutathione synthase antibody
    • Glutathione synthetase antibody
    • GSH S antibody
    • GSH synthetase antibody
    • GSH-S antibody
    • GSHB_HUMAN antibody
    • GSHS antibody
    • GSS antibody
    • HEL-S-64p antibody
    • HEL-S-88n antibody
    • MGC14098 antibody
    • OTTHUMP00000030711 antibody
    see all

Images

  • Anti-Glutathione Synthetase antibody (ab91591) at 1/100 dilution + Mouse liver tissue lysate at 35 µg

    Predicted band size : 52 kDa
  • IHC image of ab91591 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91591, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Choi WG  et al. eIF2a phosphorylation is required to prevent hepatocyte death and liver fibrosis in mice challenged with a high fructose diet. Nutr Metab (Lond) 14:48 (2017). Read more (PubMed: 28781602) »
  • Scheving R  et al. Protein S-nitrosylation and denitrosylation in the mouse spinal cord upon injury of the sciatic nerve. J Proteomics 75:3987-4004 (2012). WB, ELISA ; Mouse . Read more (PubMed: 22588120) »

See all 2 Publications for this product

Customer reviews and Q&As

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Please find the ELISA Protocol below.

Note: The precise conditions should be optimized for a particular assay.

Solution Preparation

Coating Solution:

Antigen is diluted in ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Aug 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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