Substrate for the lysosomal cysteine protease Cathepsin C. Accumulates within the lysosome. Hydrolysis by cathepsin C degrades Gly-Phe-β-naphthylamide into fragments that do not easily diffuse through the lysosomal membrane, leading to a loss of lysosome membrane integrity. Used to study intralysosomal hydrolysis, lysosomal membrane permeability, and the function of cathepsin C. Inhibits the cathepsin-dependent activation of caspase-8.
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Soluble in DMSO
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Qiu GF et al. Expression and purification of active recombinant cathepsin C (dipeptidyl aminopeptidase I) of kuruma prawn Marsupenaeus japonicus in insect cells. J Biomed Biotechnol2009:746289 (2009).
Read more (PubMed: 19707514) »
Baumgartner HK et al. Caspase-8-mediated apoptosis induced by oxidative stress is independent of the intrinsic pathway and dependent on cathepsins. Am J Physiol Gastrointest Liver Physiol293:G296-307 (2007).
Read more (PubMed: 17431216) »
Doughty MJ & Gruenstein EI Chloride-insensitive, glycine-phenylalanine-naphthylamide hydrolysis at neutral pH in human skin fibroblasts. Biochem Cell Biol64:772-81 (1986).
Read more (PubMed: 3768167) »