Anti-Glycerol 3 Phosphate Dehydrogenase antibody (ab153902)

Overview

  • Product name
    Anti-Glycerol 3 Phosphate Dehydrogenase antibody
    See all Glycerol 3 Phosphate Dehydrogenase primary antibodies
  • Description
    Rabbit polyclonal to Glycerol 3 Phosphate Dehydrogenase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 37-296 of Human Glycerol 3 Phosphate Dehydrogenase (Uniprot ID: P21695).

  • Positive control
    • HepG2, Mouse brain whole cell lysate; Human stomach tissue; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab153902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.

Target

  • Sequence similarities
    Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI747587 antibody
    • cytoplasmic antibody
    • EC 1.1.1.8 antibody
    • FLJ26652 antibody
    • G3PD antibody
    • Gdc-1 antibody
    • Gdc1 antibody
    • Gdp1 antibody
    • Glycerol 3 phosphate dehydrogenase 1 antibody
    • Glycerol 3 phosphate dehydrogenase cytosolic antibody
    • Glycerol 3 phosphate dehydrogenase soluble antibody
    • Glycerol-3-phosphate dehydrogenase [NAD+] antibody
    • Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic antibody
    • Glycerol-3-phosphate dehydrogenase 1 (soluble) antibody
    • Glycerol-3-phosphate dehydrogenase antibody
    • Glycerol-3-phosphate dehydrogenase, soluble antibody
    • Glycerphosphate dehydrogenase antibody
    • GPD-C antibody
    • gpd1 antibody
    • Gpd1 protein antibody
    • Gpd3 antibody
    • GPDA_HUMAN antibody
    • Gpdc antibody
    • GPDH antibody
    • GPDH-C antibody
    • Gpdhc antibody
    • HTGTI antibody
    • KIAA4010 antibody
    • MGC93453 antibody
    • MGPD antibody
    • mKIAA4010 antibody
    see all

Images

  • Anti-Glycerol 3 Phosphate Dehydrogenase antibody (ab153902) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 38 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Glycerol 3 Phosphate Dehydrogenase with ab153902 at 1/100 dilution.
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Glycerol 3 Phosphate Dehydrogenase with ab153902 at 1/500 dilution. Right panel co-stained with Hoechst 33343. Cells were fixed with 4% paraformaldehyde at RT for 15 minutes.
  • Anti-Glycerol 3 Phosphate Dehydrogenase antibody (ab153902) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 38 kDa



    10% SDS PAGE

References

ab153902 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Tissue lysate - whole (skeletal muscle)
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Rafael Montes Nieto

Verified customer

Submitted Apr 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up