Anti-Glycerol 3 Phosphate Dehydrogenase antibody (ab70136)

Overview

  • Product name
    Anti-Glycerol 3 Phosphate Dehydrogenase antibody
    See all Glycerol 3 Phosphate Dehydrogenase primary antibodies
  • Description
    Rabbit polyclonal to Glycerol 3 Phosphate Dehydrogenase
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Glycerol 3 Phosphate Dehydrogenase.

    (Peptide available as ab90634.)

  • Positive control
    • This antibody gave a positive signal in HepG2 whole cell lysate as well as the following tissue lysates: Human liver; Human skeletal muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab70136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Sequence similarities
    Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI747587 antibody
    • cytoplasmic antibody
    • EC 1.1.1.8 antibody
    • FLJ26652 antibody
    • G3PD antibody
    • Gdc-1 antibody
    • Gdc1 antibody
    • Gdp1 antibody
    • Glycerol 3 phosphate dehydrogenase 1 antibody
    • Glycerol 3 phosphate dehydrogenase cytosolic antibody
    • Glycerol 3 phosphate dehydrogenase soluble antibody
    • Glycerol-3-phosphate dehydrogenase [NAD+] antibody
    • Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic antibody
    • Glycerol-3-phosphate dehydrogenase 1 (soluble) antibody
    • Glycerol-3-phosphate dehydrogenase antibody
    • Glycerol-3-phosphate dehydrogenase, soluble antibody
    • Glycerphosphate dehydrogenase antibody
    • GPD-C antibody
    • gpd1 antibody
    • Gpd1 protein antibody
    • Gpd3 antibody
    • GPDA_HUMAN antibody
    • Gpdc antibody
    • GPDH antibody
    • GPDH-C antibody
    • Gpdhc antibody
    • HTGTI antibody
    • KIAA4010 antibody
    • MGC93453 antibody
    • MGPD antibody
    • mKIAA4010 antibody
    see all

Images

  • All lanes : Anti-Glycerol 3 Phosphate Dehydrogenase antibody (ab70136) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 104 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab70136 stained HeLa cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70136, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab70136 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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