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Glycerol-3-Phosphate Dehydrogenase (G3PDH) Assay Kit (Colorimetric) (ab174095) is a product where G3PDH reacts with the substrate to forms an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and rapid and can detect Glycerol-3-Phosphate dehydrogenase activity less than 1 mU/well.
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Glycerol-3-Phosphate Dehydrogenase (EC 126.96.36.199) is an important enzyme for lipid metabolism. It catalyzes the reversible conversion between dihydroxyacetone phosphate and glycerol-3-phosphate. GPDH plays multiple functions inside cells; it links carbohydrate and lipid metabolism, and provides electrons through the Glycerol-3-Phosphate Shuttle. When progenitor adipocytes differentiate into mature adipocytes, GPDH activity increases significantly. Analysis of glycerol-3-phosphate dehydrogenase activity is crucial for the study of fatty acid metabolic pathways.
|GPDH Assay Buffer||1 x 27ml|
|GPDH Positive Control||1 vial|
|GPDH Probe||1 vial|
|GPDH Substrate||1 vial|
|NADH Standard||1 vial|
Our Abpromise guarantee covers the use of ab174095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
NADH measured in cell lysates showing quantity (nmol) per 1 mln cells after 30 min incubation time.
Samples with the concentration of 5e6 cells/mL were used. Samples were diluted 2-18 fold.
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Measurement of Glycerol-3-Phosphate Dehydrogenase activity in 3T3-L1 pre- adipocyte (60 µg) and differentiated 3T3-L1 adipocytes (60 µg) using Glycerol-3-Phosphate Dehydrogenase activity (GPDH) Assay Kit (Colorimetric) (ab174095). This is example data only.
ab174095 has not yet been referenced specifically in any publications.