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Abcam’s Glycerol-3-Phosphate Assay kit (Colorimetric) (ab174094) is a sensitive, fast and easy-to-use kit. In this assay, G3P is oxidized by G3P Enzyme Mix to form an intermediate, which reduces a nearly colorless probe to a colored product with strong absorbance at 450 nm. This assay kit can detect G3P less than 2 nmol/well and can be used for a variety of sample types.
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Glycerol-3-phosphate (G3P) is an important intermediate for all living organisms. Glycerol-3-Phosphate is produced either by glycerol via glycerol kinase or by dihydroxyacetone phosphate through glycerol-3-phosphate dehydrogenase. In response to cellular signals, glycerol-3-phosphate can be utilized in multiple pathways: it can be further converted into glyceraldehyde-3-phosphate and enter glycolysis or rapidly generate NAD+ in brain or muscle tissues through the G3P shuttle or enter the lipid biosynthetic pathway. Recent studies have found that glycerol-3-phosphate is a novel regulator and plays a fundamental defense role in plant pathogenesis.
|G3P Assay Buffer||1 x 25ml|
|G3P Enzyme Mix (Lyophilized)||1 vial|
|G3P Probe||1 vial|
|G3P Standard (Lyophilized)||1 vial|
Our Abpromise guarantee covers the use of ab174094 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab174094 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"