Anti-Glycerol kinase antibody (ab70029)

Overview

  • Product nameAnti-Glycerol kinase antibody
    See all Glycerol kinase primary antibodies
  • Description
    Rabbit polyclonal to Glycerol kinase
  • Specificityab70029 detects endogenous levels of total GLPK protein.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from C-terminus of human Glycerol kinase.

  • Positive control
    • HeLa cells, extracts from 293 and Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionKey enzyme in the regulation of glycerol uptake and metabolism.
  • Tissue specificityHighly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
  • PathwayPolyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
  • Involvement in diseaseDefects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.
  • Sequence similaritiesBelongs to the FGGY kinase family.
  • Cellular localizationMitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP glycerol 3 phosphotransferase antibody
    • ATP:glycerol 3 phosphotransferase antibody
    • ATP:glycerol 3-phosphotransferase antibody
    • D930012N15Rik antibody
    • GK antibody
    • GK1 antibody
    • GKD antibody
    • GLPK_HUMAN antibody
    • Glycerokinase antibody
    • Glycerol kinase antibody
    • OTTHUMP00000023108 antibody
    • OTTHUMP00000023109 antibody
    • OTTHUMP00000215321 antibody
    see all

Anti-Glycerol kinase antibody images

  • All lanes : Anti-Glycerol kinase antibody (ab70029) at 1/500 dilution

    Lane 1 : extracts from 293 cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from 293 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • This image shows HeLa cells with ab70029 at 1/500 dilution, with and without the immunising peptide.

References for Anti-Glycerol kinase antibody (ab70029)

ab70029 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Mouse Cell (Adipocytes)
Specification Adipocytes
Permeabilization Yes - pbst 0.2% triton x-100
Fixative Paraformaldehyde
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Submitted Sep 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"