Anti-Glycerol kinase antibody (ab70029)

Overview

  • Product name
    Anti-Glycerol kinase antibody
    See all Glycerol kinase primary antibodies
  • Description
    Rabbit polyclonal to Glycerol kinase
  • Specificity
    ab70029 detects endogenous levels of total GLPK protein.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from C-terminus of human Glycerol kinase.

  • Positive control
    • HeLa cells, extracts from 293 and Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • Function
    Key enzyme in the regulation of glycerol uptake and metabolism.
  • Tissue specificity
    Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
  • Pathway
    Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
  • Involvement in disease
    Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.
  • Sequence similarities
    Belongs to the FGGY kinase family.
  • Cellular localization
    Mitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP glycerol 3 phosphotransferase antibody
    • ATP:glycerol 3 phosphotransferase antibody
    • ATP:glycerol 3-phosphotransferase antibody
    • D930012N15Rik antibody
    • GK antibody
    • GK1 antibody
    • GKD antibody
    • GLPK_HUMAN antibody
    • Glycerokinase antibody
    • Glycerol kinase antibody
    • OTTHUMP00000023108 antibody
    • OTTHUMP00000023109 antibody
    • OTTHUMP00000215321 antibody
    see all

Images

  • All lanes : Anti-Glycerol kinase antibody (ab70029) at 1/500 dilution

    Lane 1 : extracts from 293 cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from 293 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • This image shows HeLa cells with ab70029 at 1/500 dilution, with and without the immunising peptide.

References

ab70029 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Mouse Cell (Adipocytes)
Specification
Adipocytes
Permeabilization
Yes - pbst 0.2% triton x-100
Fixative
Paraformaldehyde
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Submitted Sep 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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