Anti-Glycerol kinase antibody (ab88505)

Overview

  • Product nameAnti-Glycerol kinase antibody
    See all Glycerol kinase primary antibodies
  • Description
    Mouse polyclonal to Glycerol kinase
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-524 of Human Glycerol kinase (NP_000158.1)

  • Positive control
    • human placenta lysate, transfected 293T cell line, human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab88505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesIHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 57 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionKey enzyme in the regulation of glycerol uptake and metabolism.
    • Tissue specificityHighly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
    • PathwayPolyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
    • Involvement in diseaseDefects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.
    • Sequence similaritiesBelongs to the FGGY kinase family.
    • Cellular localizationMitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP glycerol 3 phosphotransferase antibody
      • ATP:glycerol 3 phosphotransferase antibody
      • ATP:glycerol 3-phosphotransferase antibody
      • D930012N15Rik antibody
      • GK antibody
      • GK1 antibody
      • GKD antibody
      • GLPK_HUMAN antibody
      • Glycerokinase antibody
      • Glycerol kinase antibody
      • OTTHUMP00000023108 antibody
      • OTTHUMP00000023109 antibody
      • OTTHUMP00000215321 antibody
      see all

    Anti-Glycerol kinase antibody images

    • Anti-Glycerol kinase antibody (ab88505) at 5 µg/ml + human placenta lysate at 50 µg

      Secondary
      HRP goat anti mouse IgG at 1/2500 dilution

      Predicted band size : 57 kDa
    • All lanes : Anti-Glycerol kinase antibody (ab88505) at 5 µg/ml

      Lane 1 : transfected 293T cell line
      Lane 2 : non transfected 293T cell line

      Lysates/proteins at 25 µg per lane.

      Secondary
      HRP goat anti mouse IgG at 1/2500 dilution

      Predicted band size : 57 kDa
    • Immunohistochemical analysis of Glycerol kinase expression in formalin-fixed paraffin-embedded human kidney using 3ug ab88505.

    References for Anti-Glycerol kinase antibody (ab88505)

    ab88505 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 22°C
    Antigen retrieval step Heat mediated
    Sample Mouse Tissue sections (Adipose tissue)
    Specification Adipose tissue
    Permeabilization Yes - PBS 0.5% TritonX-100
    Fixative Paraformaldehyde
    Username

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    Verified customer

    Submitted Dec 18 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"