Anti-Glycerol kinase antibody (Biotin) (ab34535)

Overview

  • Product nameAnti-Glycerol kinase antibody (Biotin)
    See all Glycerol kinase primary antibodies
  • Description
    Goat polyclonal to Glycerol kinase (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, ELISA, Immunomicroscopy, Dot blotmore details
  • Species reactivity
    Reacts with: Goat
  • Immunogen

    Glycerol kinase, Cellulomonas species.

  • General notes

    10-20 Biotin molecules per goat IgG molecule.

Properties

Applications

Our Abpromise guarantee covers the use of ab34535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Immunomicroscopy
Dot blot
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/20000.
    This product has been assayed against 1.0µg of Glycerol kinase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic-acid]) as a substrate for 30 minutes at RT.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 57 kDa.
    Suitable for other antibody based assays using streptavidin or avidin conjugates.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionKey enzyme in the regulation of glycerol uptake and metabolism.
    • Tissue specificityHighly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
    • PathwayPolyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
    • Involvement in diseaseDefects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.
    • Sequence similaritiesBelongs to the FGGY kinase family.
    • Cellular localizationMitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
    • Information by UniProt
    • Alternative names
      • ATP glycerol 3 phosphotransferase antibody
      • ATP:glycerol 3 phosphotransferase antibody
      • ATP:glycerol 3-phosphotransferase antibody
      • D930012N15Rik antibody
      • GK antibody
      • GK1 antibody
      • GKD antibody
      • GLPK_HUMAN antibody
      • Glycerokinase antibody
      • Glycerol kinase antibody
      • OTTHUMP00000023108 antibody
      • OTTHUMP00000023109 antibody
      • OTTHUMP00000215321 antibody
      see all

    References for Anti-Glycerol kinase antibody (Biotin) (ab34535)

    ab34535 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"