Recombinant
RabMAb

Anti-Glycerol kinase antibody [EPR6567] (ab126599)

Overview

  • Product name
    Anti-Glycerol kinase antibody [EPR6567]
    See all Glycerol kinase primary antibodies
  • Description
    Rabbit monoclonal [EPR6567] to Glycerol kinase
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Fetal liver and HepG2 cell lysates. Permeabilized HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab126599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      Key enzyme in the regulation of glycerol uptake and metabolism.
    • Tissue specificity
      Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
    • Pathway
      Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
    • Involvement in disease
      Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.
    • Sequence similarities
      Belongs to the FGGY kinase family.
    • Cellular localization
      Mitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP glycerol 3 phosphotransferase antibody
      • ATP:glycerol 3 phosphotransferase antibody
      • ATP:glycerol 3-phosphotransferase antibody
      • D930012N15Rik antibody
      • GK antibody
      • GK1 antibody
      • GKD antibody
      • GLPK_HUMAN antibody
      • Glycerokinase antibody
      • Glycerol kinase antibody
      • OTTHUMP00000023108 antibody
      • OTTHUMP00000023109 antibody
      • OTTHUMP00000215321 antibody
      see all

    Images

    • All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/1000 dilution

      Lane 1 : Mouse adipose tissue lysate
      Lane 2 : Mouse adipose tissue lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      HRP-conjugated Donkey anti-rabbit polyclonal at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 61 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes

      This image is courtesy of an anonymous Abreview

      See Abreview

    • All lanes : Anti-Glycerol kinase antibody [EPR6567] (ab126599) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 61 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    • ab126599, at 1/100 dilution, staining Glycerol kinase in permeabilized HepG2 cells by Immunofluorescence (red). A rabbit IgG is used as a negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Mottillo EP  et al. Coupling of lipolysis and de novo lipogenesis in brown, beige, and white adipose tissues during chronic ß3-adrenergic receptor activation. J Lipid Res 55:2276-86 (2014). Read more (PubMed: 25193997) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Sample
    Mouse Tissue lysate - whole (Adipose tissue lysate)
    Specification
    Adipose tissue lysate
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 02 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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