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Products:Neuroscience >> Neurotransmitter >> Amino Acids >> Glycine
Overview
Product name
Anti-Glycine antibody
See all Glycine products (2) ...
Description
Rabbit polyclonal to Glycine
Specificity
The antibody is calibrated against a spectrum of antigens to assure hapten selectivity. No measurable cross-reactivity (<1:1000) was detected against glycine in peptides or proteins. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine.
Tested applications
IHC-FoFr, IHC-Fr, ICC, Immunomicroscopymore details
Cross reactivity
Reacts with
Rat
Immunogen
Chemical / Small Molecule by a Glutaraldehyde linker.
General notes
The product is optimized for HPI/EHPI with gold or fluorescence detection using etched plastic sections. Filter diluted reagents with 0.2 mm syringe filters before use on EM grids. Enzyme-linked visualizations can be used but will compress the signal dynamic range and are less sensitive.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurotransmitter >> Amino Acids >> Glycine
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution. (Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur. Use in whole mounts is not recommended for similar reasons.)
IHC-Fr: Use at an assay dependent dilution.
ICC: 1/100(See protocol. OPTIMAL FIXATION: 0.5-2.5% glutaraldehyde,1% formaldehyde using HPI protocol (The antisera targets the glutaraldehyde conjugate of the hapten). MINIMAL GLUTARALDEHYDE: 0.1% using HPI All procedures may be carried out at room temperature. Exact dilutions for all applications cannot be predicted, but it is unlikely that deviations from the calibrated levels will be needed. Dilutions are optimized for antigen detection over a 2 log unit range.)
IM: Use at an assay dependent dilution.
Target
Relevance
Defects in GLDC are a cause of nonketotic hyperglycinemia (NKH), also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms. The degredation of glycine is catalised by the glycine cleavage system. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; carbondioxide is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. The glycine cleavage system is composed of four proteins: P, T, L and H.
References for Anti-Glycine antibody (ab9442)
This product has been referenced in:
- Sun Det al. Metabolic and functional profiling of the normal rat retina. J Comp Neurol 505:92-113 (2007). IHC; Rat.Read more (PubMed: 17729258) »
See 1 publication for this product
Publishing research using ab9442? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"