Glycine antibody (ab9442)

Product Name 

Glycine antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Glycine

Immunogen 

Chemical / Small Molecule by a Glutaraldehyde linker.

Reacts with 
(species key)

Rat

Specificity 

The antibody is calibrated against a spectrum of antigens to assure hapten selectivity.

No measurable cross-reactivity (<1:1000) was detected against glycine in peptides or proteins.

Fixed tissue cross-reactivity was tested with known targets at the recommended dilution.
No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine.

Tested applications 
(see key)

ICC, IHC-FoFr, IHC-Fr, IM

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
This antibody is recommended for thin section post-embedding endogenous content mapping by Light and Electron microscopy immunostaining via High Performance Immunocytochemistry (HPI) (see "Protocols" tab above)

Recommended user dilution: 1/100
True Dilution at User Dilution: 1/2000

High-performance thin section immunostaining using silver-intensified immunogold or fluorescence detection.
Enhanced detection is possible using streptavidin detection.
EM applications with 10-40 nm gold GAR IgGs or gold streptavidin.

OPTIMAL FIXATION: 0.5-2.5% glutaraldehyde,1% formaldehyde using HPI protocol (The antisera targets the glutaraldehyde conjugate of the hapten).
MINIMAL GLUTARALDEHYDE: 0.1% using HPI

The hapten is osmium tolerant (deosmication required), therefore the antisera can be used on conventional post-embedding electron microscope immunostaining.

All procedures may be carried out at room temperature. Exact dilutions for all applications cannot be predicted, but it is unlikely that deviations from the calibrated levels will be needed. Dilutions are optimized for antigen detection over a 2 log unit range.

The product is optimized for HPI/EHPI with gold or fluorescence detection using etched plastic sections. Filter diluted reagents with 0.2 mm syringe filters before use on EM grids. Enzyme-linked visualizations can be used but will compress the signal dynamic range and are less sensitive.

Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur. Use in whole mounts is not recommended for similar reasons.

Research areas 

Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurotransmitter >> Amino Acids >> Glycine

Relevance 

Defects in GLDC are a cause of nonketotic hyperglycinemia (NKH), also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms.
The degredation of glycine is catalised by the glycine cleavage system. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; carbondioxide is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.
The glycine cleavage system is composed of four proteins: P, T, L and H.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

n/a

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C. Do not freeze.

References for Glycine antibody (ab9442)

  This product has been used in:

Sun D et al. Metabolic and functional profiling of the normal rat retina. J Comp Neurol 505:92-113 (2007). IHC; Rat. PubMed: 17729258

If you publish research using ab9442 please let us know so that we can cite the reference on this datasheet.

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