• Product nameAnti-Glycine antibody
    See all Glycine primary antibodies
  • Description
    Rabbit polyclonal to Glycine
  • SpecificityThe antibody is calibrated against a spectrum of antigens to assure hapten selectivity. No measurable cross-reactivity (<1:1000) was detected against glycine in peptides or proteins. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine.
  • Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, Immunomicroscopymore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule by a Glutaraldehyde linker.

  • General notesThe product is optimized for HPI/EHPI with gold or fluorescence detection using etched plastic sections. Filter diluted reagents with 0.2 mm syringe filters before use on EM grids. Enzyme-linked visualizations can be used but will compress the signal dynamic range and are less sensitive.



Our Abpromise guarantee covers the use of ab9442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur. Use in whole mounts is not recommended for similar reasons.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.


  • RelevanceDefects in GLDC are a cause of nonketotic hyperglycinemia (NKH), also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms. The degredation of glycine is catalised by the glycine cleavage system. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; carbondioxide is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. The glycine cleavage system is composed of four proteins: P, T, L and H.

References for Anti-Glycine antibody (ab9442)

This product has been referenced in:
  • Fredrich M  et al. Neuronal subtype identity in the rat auditory brainstem as defined by molecular profile and axonal projection. Exp Brain Res 195:241-60 (2009). Read more (PubMed: 19340418) »
  • Sun D  et al. Metabolic and functional profiling of the normal rat retina. J Comp Neurol 505:92-113 (2007). IHC . Read more (PubMed: 17729258) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Sample Chicken Tissue sections (chicken hindbrain)
Specification chicken hindbrain
Permeabilization Yes - 0.1% PBS-TritonX
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 26 2014

Thank you for contacting Abcam.

1. Bovine calf serum rather than goat serum should work to sufficiently block non-specific binding.

2) As stated on the datasheet, this antibody is tested and guaranteed to react with rat Glycine via IH...

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Thank you for contacting us. We have anti glycine or anti- proline antibodies in our catalogue. The catalogue numbers are ab9344, ab9345, ab37067, ab9442 and ab37087. We unfortunately do not have antibodies available specifically raised against ...

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Thank you for your enquiry. This product is sold as whole antiserum, not as purified antibody. An exact concentration is not available. In general, we estimate that the total Ig concentration in serum is approx. 10 mg/ml, and the specific antibody comp...

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Thank you for your reply and for your interesting comment. We have looked at your article very carefully. This is a 4% PF fixation which does not cross-link tissue. That is why the penetration is better - but not good. Most anti-glycine antibod...

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Thank you for your enquiry and for providing more details of your protocol. As we understand from your e-mail, the main problem is that you have no glycine signals. This is very likely a technical failure. Reading through your protocol, it is clear ...

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Dear George 1) This antibody should work with 0.1% glutaraldehyde fixation using the High Performance Immunocytochemistry protocol listed with the antibody on the Abcam website. 2) I have had a search on the web and I have learned from this t...

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