Anti-Glycine Receptor alpha 1 + alpha 2 antibody (ab23809)

Overview

  • Product name
    Anti-Glycine Receptor alpha 1 + alpha 2 antibody
  • Description
    Rabbit polyclonal to Glycine Receptor alpha 1 + alpha 2
  • Specificity
    This product recognises an epitope within the N-terminal region of glycine receptor alpha 1 and alpha 2 subunits.
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (N-terminal) (Rat).

  • Positive control
    • Rat brain lysate, rat spinal cord lysate.
  • General notes
    For long term storage the addition of 0.09% sodium azide is recommended.

Properties

Applications

Our Abpromise guarantee covers the use of ab23809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/1000.
WB 1/1000. Detects a band of approximately 48 kDa.
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/1000 for overnight incubation on rat cells (see Abreview submitted by Johana Trojanova).
IHC-FoFr Use at an assay dependent concentration. PubMed: 21215254

Target

Images

  •     ab23809 at a 1/1000 dilution staining rat brain stem cells(medial nucleus of trapezoid body) by ICC/IF. The cells were fixed with paraformaldehyde and incubated with the antibody for 18 hours. An Alexa Fluor ® 488 donkey anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab23809 staining Glycin Receptor alpha1 + alpha 2 in Mouse retinal tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/400 in PBST) for 12 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Ren M  et al. A biofidelic 3D culture model to study the development of brain cellular systems. Sci Rep 6:24953 (2016). ICC/IF ; Rat . Read more (PubMed: 27112667) »
  • Yoo YB  et al. Changes in the immunohistochemical localization of the glycine receptor in the superior olivary complex of adult circling mice. Mol Med Rep 12:7927-32 (2015). IHC . Read more (PubMed: 26498980) »

See all 10 Publications for this product

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse retina sections)
Specification
mouse retina sections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Kevin Jin

Verified customer

Submitted Nov 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Brain Stem, Medial Nucleus of Trapezoid Body,)
Specification
Brain Stem, Medial Nucleus of Trapezoid Body,
Fixative
Paraformaldehyde
Blocking step
Other as blocking agent for 16 hour(s) and 1 minute(s) · Concentration: 5%
Username

Ms. Johana Trojanova

Verified customer

Submitted Sep 08 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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