Anti-Glycogen synthase 1 antibody [EP817Y] (ab40810)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab40810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa).
ICC/IF 1/250 - 1/500.
Flow Cyt 1/50 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

Target

  • FunctionTransfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
  • PathwayGlycan biosynthesis; glycogen biosynthesis.
  • Involvement in diseaseDefects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
  • Sequence similaritiesBelongs to the glycosyltransferase 3 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glycogen [starch] synthase antibody
    • Glycogen synthase 1 (muscle) antibody
    • Glycogen synthase 1 antibody
    • GSY antibody
    • GYS antibody
    • Gys1 antibody
    • GYS1_HUMAN antibody
    • muscle antibody
    see all

Anti-Glycogen synthase 1 antibody [EP817Y] images

  • Overlay histogram showing HEK293 cells stained with ab40810 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40810, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-Glycogen synthase 1 antibody [EP817Y] (ab40810) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Predicted band size : 81 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  • ab40810 at a 1:250 dilution staining Hela cells using anti-Glycogen Synthase 1 RabMAb.

References for Anti-Glycogen synthase 1 antibody [EP817Y] (ab40810)

This product has been referenced in:
  • Rubio-Villena C  et al. Glycogenic activity of R6, a protein phosphatase 1 regulatory subunit, is modulated by the laforin-malin complex. Int J Biochem Cell Biol 45:1479-88 (2013). Read more (PubMed: 23624058) »
  • Zhang Y  et al. Regulation of lipid and glucose homeostasis by mango tree leaf extract is mediated by AMPK and PI3K/AKT signaling pathways. Food Chem 141:2896-905 (2013). WB ; Mouse . Read more (PubMed: 23871039) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (A549 Lung cancer cells)
Specification A549 Lung cancer cells
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Mar 04 2015

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 14 µg
Gel Running Conditions Reduced Denaturing (4-20% Tris Glycin)
Sample Mouse Tissue lysate - whole (Adipose tissue)
Specification Adipose tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 21 2013

The immunogen sequence is within the last 25 amino acids of the C terminus of human GYS1. Yes, a single amino acid difference within the sequence that defines the epitope will be enough to reduce binding affinity, often to the point where there is no s...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Placentas)
Specification Placentas
Fixative Buffered Formalin
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"