Recombinant
RabMAb

Anti-Glycogen synthase 1 antibody [EP817Y] (ab40810)

Overview

  • Product name
    Anti-Glycogen synthase 1 antibody [EP817Y]
    See all Glycogen synthase 1 primary antibodies
  • Description
    Rabbit monoclonal [EP817Y] to Glycogen synthase 1
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Human Glycogen Synthase.

  • Positive control
    • Hela cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab40810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa).
ICC/IF 1/250 - 1/500.
Flow Cyt 1/50 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

Target

  • Function
    Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
  • Pathway
    Glycan biosynthesis; glycogen biosynthesis.
  • Involvement in disease
    Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
  • Sequence similarities
    Belongs to the glycosyltransferase 3 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glycogen [starch] synthase antibody
    • Glycogen synthase 1 (muscle) antibody
    • Glycogen synthase 1 antibody
    • GSY antibody
    • GYS antibody
    • Gys1 antibody
    • GYS1_HUMAN antibody
    • muscle antibody
    see all

Images

  • Overlay histogram showing HEK293 cells stained with ab40810 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40810, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-Glycogen synthase 1 antibody [EP817Y] (ab40810) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Predicted band size : 81 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  • ab40810 at a 1:250 dilution staining Hela cells using anti-Glycogen Synthase 1 RabMAb.

References

This product has been referenced in:
  • Zhao Y  et al. Decreased Glycogen Content Might Contribute to Chronic Stress-Induced Atrophy of Hippocampal Astrocyte volume and Depression-like Behavior in Rats. Sci Rep 7:43192 (2017). Read more (PubMed: 28233800) »
  • Xirouchaki CE  et al. Impaired glucose metabolism and exercise capacity with muscle-specific glycogen synthase 1 (gys1) deletion in adult mice. Mol Metab 5:221-32 (2016). Read more (PubMed: 26977394) »

See all 6 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (A549 Lung cancer cells)
Specification
A549 Lung cancer cells
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Mar 04 2015

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
14 µg
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin)
Sample
Mouse Tissue lysate - whole (Adipose tissue)
Specification
Adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 21 2013

The immunogen sequence is within the last 25 amino acids of the C terminus of human GYS1. Yes, a single amino acid difference within the sequence that defines the epitope will be enough to reduce binding affinity, often to the point where there is no s...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Placentas)
Specification
Placentas
Fixative
Buffered Formalin
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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