Human muscle glycogen synthase (GYS1) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GYS1 is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GYS1 at Ser 640. Phosphorylation of GYS1 at S640 has been associated with Antiphospholipid Antibody Syndrome.
Our Abpromise guarantee covers the use of ab2479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation.
Immunohistochemistry with Anti-Glycogen Synthase antibody Tissue: Human Prostate Fixation: formalin-fixed, paraffin-embedded tissue Antigen retrieval: heat-induced Primary antibody(ab2479): 5 µg/ml Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
Rabbit polyclonal to phospho Glycogen Synthase (Ser 640) used at a 1/1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 mM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'.
Goat polyclonal to rabbit IgG (HRP) (ab7090) was used as secondary antibody at 1/5000.A 4-20% Criterion gel for SDS-PAGE was used.
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