Anti-Glycogenin 2 antibody (ab204784)
Key features and details
- Rabbit polyclonal to Glycogenin 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glycogenin 2 antibody
See all Glycogenin 2 primary antibodies -
Description
Rabbit polyclonal to Glycogenin 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Glycogenin 2 aa 350-500.
Database link: O15488 -
Positive control
- Human liver tissue; RT-4 and U-251 MG cell lysates; SK-MEL-30 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 55 kDa.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 55 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 8908 Human
- Omim: 300198 Human
- SwissProt: O15488 Human
- Unigene: 567381 Human
- Unigene: 701629 Human
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Alternative names
- Glycogenin 2 antibody
- Glycogenin glucosyltransferase antibody
- Glycogenin-2 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling Glycogenin 2 with ab204784 at 1/1000 dilution.
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Immunofluorescent analysis of SK-MEL-30 cells (PFA-fixed/Triton X-100 permeabilized) labeling Glycogenin 2 with ab204784 at 4 µg/ml (green).
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All lanes : Anti-Glycogenin 2 antibody (ab204784) at 1/500 dilution
Lane 1 : RT-4 cell lysates
Lane 2 : U-251 MG cell lysates
Developed using the ECL technique.
Predicted band size: 55 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab204784 has been referenced in 1 publication.
- Noguchi Y et al. Microscopic image-based covariation network analysis for actin scaffold-modified insulin signaling. iScience 24:102724 (2021). PubMed: 34337357