• Product nameAnti-Glycophorin A antibody [JC159]
    See all Glycophorin A primary antibodies
  • Description
    Mouse monoclonal [JC159] to Glycophorin A
  • SpecificityThis antibody is specific to erythroid cells at various stages of differentiation from the erythroblast to the mature red cell.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Formalin fixed membrane from a Hairy cell leukemia.

  • Positive control
    • Tonsil



Our Abpromise guarantee covers the use of ab9520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • FunctionGlycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
  • Sequence similaritiesBelongs to the glycophorin A family.
  • Post-translational
    The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
  • Cellular localizationCell membrane. Appears to be colocalized with SLC4A1.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI853584 antibody
    • Blood group--MN locus antibody
    • CD_antigen=CD235a antibody
    • CD235a antibody
    • GLPA_HUMAN antibody
    • Glycophorin A (MNS blood group) antibody
    • Glycophorin A antibody
    • Glycophorin A, included antibody
    • Glycophorin-A antibody
    • GlycophorinA antibody
    • GPA antibody
    • GPErik antibody
    • GpMiIII antibody
    • GPSAT antibody
    • GYPA antibody
    • GYPA, included antibody
    • HGpMiIII antibody
    • HGpMiV antibody
    • HGpMiX antibody
    • HGpMiXI antibody
    • HGpSta(C) antibody
    • MN antibody
    • MN sialoglycoprotein antibody
    • MNS antibody
    • PAS-2 antibody
    • PAS2 antibody
    • Sialoglycoprotein alpha antibody
    see all

Anti-Glycophorin A antibody [JC159] images

  • Ab9520 staining Human lung. Staining is localised to red blood cells in lung tissue.
    Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining Glycophorin A with ab9520.

References for Anti-Glycophorin A antibody [JC159] (ab9520)

This product has been referenced in:
  • Estarás C  et al. Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program. Development 139:2681-91 (2012). Read more (PubMed: 22782721) »

See 1 Publication for this product

Product Wall

Thank you for your reply. In general, most antibodies have some degree of non-specific binding. For flow cytometry, I can recommend BSA or FCS recommend in staining and wash buffers. Some samples (e.g. cells with Fc receptors li...

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Thank you for your inquiry. As my colleague mentioned in the previous email, this antibody is not tested and guaranteed for flow cytometry. Unfortunately, we do not have any information about its suitability for flow cytometry. Suitable ...

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Thank you for your enquiry and your interest in our products. Generally, if the antibody is provided as ascitic fluid and not affinity purified product; we do not determine the IgG concentration. Ab9520 is an exemption, the protein concentration ...

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