The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/25.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
1/100 - 1/1000.
This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
1/100 - 1/1000.
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
Belongs to the glycophorin A family.
The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
Cell membrane. Appears to be colocalized with SLC4A1.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [YTH89.1] (ab33386)
ab33386 staining erythrocytes in sinusoids of human hypophysis by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.
Flow Cytometry - Anti-Glycophorin A antibody [YTH89.1] (ab33386)This image is courtesy of an Abreview submitted by Mark Allenby
ab33386 staining Glycophorin A in isolated human cord blood by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 1% BSA + 0.1% Triton X-100 + 0.05% Tween-20 + 0.01% NaN3. The sample was incubated with the primary antibody (2µg/ml in !% BSA + 0.05% Tween-20 + 0.01% NaN3) for 2 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rat IgG polyclonal (1/1000) was used as the secondary antibody. Gating Strategy: Against debris (low SSC/high FSC).