Anti-Glycophorin A + B antibody [HIR2] (Biotin) (ab93548)


  • Product nameAnti-Glycophorin A + B antibody [HIR2] (Biotin)
    See all Glycophorin A + B primary antibodies
  • Description
    Mouse monoclonal [HIR2] to Glycophorin A + B (Biotin)
  • ConjugationBiotin
  • SpecificityReacts with Human Glycophorin A and B, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Glycophorin A + B (N terminal).

  • Positive control
    • Human peripheral blood leukocytes
  • General notesMouse monoclonal [HIR2] to Glycophorin A (Biotin) also has a clone number GA-R2.

    Binding of ab93548 to red cells at high antibody concentration causes cell agglutination.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesBinding of ab93548 to red cells at high antibody concentration causes cell agglutination.
  • ClonalityMonoclonal
  • Clone numberHIR2
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab93548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.125µg for 105 cells.

Use 0.125µg for 105 to 108 cells in a final volume of 100 µl.

ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • RelevanceGlycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
  • Cellular localizationType I membrane protein.
  • Database links
  • Alternative names
    • CD235a antibody
    • CD235b antibody
    • Erythroid lineage specific membrane sialoglycoprotein antibody
    • Glycophorin Erik antibody
    • Glycophorin HeP2 antibody
    • Glycophorin MiI antibody
    • Glycophorin MiIII antibody
    • Glycophorin MiV antibody
    • Glycophorin MiVI antibody
    • Glycophorin MiX antibody
    • Glycophorin SAT antibody
    • Glycophorin Sta type C antibody
    • GPA antibody
    • GPB antibody
    • GPB.NY antibody
    • GPErik antibody
    • GpMiIII antibody
    • GPSAT antibody
    • GYPA antibody
    • GYPB antibody
    • GYPHe.NY antibody
    • HGpMiIII antibody
    • HGpMiV antibody
    • HGpMiVI antibody
    • HGpMiX antibody
    • HGpMiXI antibody
    • HGpSta(C) antibody
    • Mi.V glycoprotein (24 AA) antibody
    • MN antibody
    • MN sialoglycoprotein antibody
    • MNS antibody
    • PAS 2 antibody
    • PAS 3 antibody
    • PAS2 antibody
    • PAS3 antibody
    • Sialoglycoprotein alpha antibody
    • Sialoglycoprotein delta antibody
    • SS active sialoglycoprotein antibody
    • SS antibody
    see all

References for Anti-Glycophorin A + B antibody [HIR2] (Biotin) (ab93548)

This product has been referenced in:
  • Chen G  et al. [Immunophenotyping study on the blood cells of the patients with paroxysmal nocturnal hemoglobinuria] Zhonghua Xue Ye Xue Za Zhi 18:190-2 (1997). Read more (PubMed: 15622765) »

See 1 Publication for this product

Product Wall

Thank you for contacting us with your questions, and for your patience while I have been in touch with the lab for more information about ab93548.

The lab has informed me that there are approximately 3-7 molecules of biotin per antibody.The c...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Permeabilization No

Dr. Elodie Segura

Verified customer

Submitted Jan 25 2011