Anti-Glycophorin A + B antibody [HIR2] (FITC) (ab52452)

Overview

  • Product name
    Anti-Glycophorin A + B antibody [HIR2] (FITC)
    See all Glycophorin A + B primary antibodies
  • Description
    Mouse monoclonal [HIR2] to Glycophorin A + B (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    ab52452 recognises N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins expressed on red blood cells and erythroid precursor cells.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Glycophorin A + B (N terminal).

  • General notes

    ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    Prior to conjugation, ab52452 was purified by Protein G affinity chromatography. After conjugation, it was purified by site exclusion chromatography.
  • Primary antibody notes
    ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
  • Clonality
    Monoclonal
  • Clone number
    HIR2
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 20µl for 106 cells.

Use 20µl for 106 cells, or for 100 µl of whole blood.





ab91368-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPA gene consists of 7 exons and has 97% sequence homology with GYPB from the 5' UTR to the coding sequence encoding the first 45 amino acids. GYPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are resulted from gene recombinations between GYPA and GYPB. These antigens are expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
  • Cellular localization
    Type I membrane protein.
  • Database links
  • Alternative names
    • CD235a antibody
    • CD235b antibody
    • Erythroid lineage specific membrane sialoglycoprotein antibody
    • Glycophorin Erik antibody
    • Glycophorin HeP2 antibody
    • Glycophorin MiI antibody
    • Glycophorin MiIII antibody
    • Glycophorin MiV antibody
    • Glycophorin MiVI antibody
    • Glycophorin MiX antibody
    • Glycophorin SAT antibody
    • Glycophorin Sta type C antibody
    • GPA antibody
    • GPB antibody
    • GPB.NY antibody
    • GPErik antibody
    • GpMiIII antibody
    • GPSAT antibody
    • GYPA antibody
    • GYPB antibody
    • GYPHe.NY antibody
    • HGpMiIII antibody
    • HGpMiV antibody
    • HGpMiVI antibody
    • HGpMiX antibody
    • HGpMiXI antibody
    • HGpSta(C) antibody
    • Mi.V glycoprotein (24 AA) antibody
    • MN antibody
    • MN sialoglycoprotein antibody
    • MNS antibody
    • PAS 2 antibody
    • PAS 3 antibody
    • PAS2 antibody
    • PAS3 antibody
    • Sialoglycoprotein alpha antibody
    • Sialoglycoprotein delta antibody
    • SS active sialoglycoprotein antibody
    • SS antibody
    see all

References

ab52452 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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