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Synthetic peptide corresponding to Human Glycophorin A + B (N terminal).
ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
Our Abpromise guarantee covers the use of ab52452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 20µl for 106 cells.
Use 20µl for 106 cells, or for 100 µl of whole blood.
ab91368-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ab52452 has not yet been referenced specifically in any publications.