Anti-Glycoprotein hormones (alpha 5) antibody [EP3373] (ab92738)

Overview

  • Product nameAnti-Glycoprotein hormones (alpha 5) antibody [EP3373]
  • Description
    Rabbit monoclonal [EP3373] to Glycoprotein hormones (alpha 5)
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Glycoprotein hormones (alpha 5) aa 1-100.
    Database link: P01215

  • Positive control
    • JAR cell lysates treated with Hydroxyurea; Human placenta tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab92738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/2000.

For unpurified, use 1/100 - 1/250.

Target

  • RelevanceThe four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The alpha subunit belongs to the glycoprotein hormones alpha chain family.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Anterior pituitary glycoprotein hormones common subunit alpha antibody
    • CG alpha antibody
    • CGA antibody
    • Choriogonadotropin alpha chain antibody
    • Chorionic gonadotrophin subunit alpha antibody
    • Chorionic gonadotropin, alpha polypeptide antibody
    • Follicle stimulating hormone alpha chain antibody
    • Follicle stimulating hormone alpha subunit antibody
    • Follitropin alpha chain antibody
    • FSH alpha antibody
    • Glycoprotein hormones alpha chain antibody
    • Glycoprotein hormones, alpha polypeptide antibody
    • LSH alpha antibody
    • Luteinizing hormone alpha chain antibody
    • Lutropin alpha chain antibody
    • Pituitary hormone antibody
    • Thyroid stimulating hormone alpha chain antibody
    • Thyrotropin alpha chain antibody
    • TSH alpha antibody
    see all

Anti-Glycoprotein hormones (alpha 5) antibody [EP3373] images

  • Anti-Glycoprotein hormones (alpha 5) antibody [EP3373] (ab92738) at 1/5000 dilution (purified) + human placenta at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 13 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-Glycoprotein hormones (alpha 5) antibody [EP3373] (ab92738) at 1/1000 dilution (purified) + Hydroxyurea treated JAR whole cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 13 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human placenta with purified ab92738 at a working dilution of 1/2000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human pancreas with purified ab92738 at a working dilution of 1/2000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • All lanes : Anti-Glycoprotein hormones (alpha 5) antibody [EP3373] (ab92738) at 1/500 dilution (unpurified)

    Lane 1 : JAR cell lysates, treated with Hydroxyurea
    Lane 2 : JAR cell lysates, untreated

    Lysates/proteins at 10 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 13 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
  • Unpurified ab92738 showing negative staining in Normal breast tissue.

  • Unpurified ab92738 showing negative staining in Normal kidney tissue.

References for Anti-Glycoprotein hormones (alpha 5) antibody [EP3373] (ab92738)

ab92738 has not yet been referenced specifically in any publications.

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