Overview

  • Product nameAnti-GLYCTK antibody
    See all GLYCTK primary antibodies
  • Description
    Mouse monoclonal to GLYCTK
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cynomolgus Monkey
  • Immunogen

    Recombinant fragment: VLSAAMQGDV KSMAQFYGLL AHVARTRLTP SMAGASVEED AQLHELAAEL QIPDLQLEEA LETMAWGRGP VCLLAGGEPT VQLQGSGRGG RNQELALRV, corresponding to amino acids 332-430 of human GLYCTK (NP_660305) with a 26 kDa tag

  • Positive control
    • NIH/3T3 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 55 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Tissue specificityWidely expressed.
  • Involvement in diseaseDefects in GLYCTK are the cause of D-glyceric aciduria (D-GA) [MIM:220120]. D-GA is a rare metabolic disease characterized by chronic metabolic acidosis and a highly variable clinical phenotype. Clinical features range from an encephalopathic presentation with seizures, microcephaly, severe mental retardation and early death, to milder manifestations with only speech delay or even normal development.
  • Sequence similaritiesBelongs to the glycerate kinase type-2 family.
  • Cellular localizationCytoplasm and Cytoplasm. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • CG9886 like antibody
    • GLCTK_HUMAN antibody
    • Glycerate kinase antibody
    • Glyctk antibody
    • GLYCTK1 antibody
    • HBeAg binding protein 2 antibody
    • HBeAg binding protein 4 antibody
    • HBeAg-binding protein 4 antibody
    • HBeAgBP4A antibody
    • HBEBP2 antibody
    • HBEBP4 antibody
    see all

Anti-GLYCTK antibody images

  • Anti-GLYCTK antibody (ab77727) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 55 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-GLYCTK antibody (ab77727) at 5 µg/ml + NIH/3T3 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 55 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-GLYCTK antibody (ab77727)

ab77727 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77727.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"