The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceGLYL2 belongs to the glycine N acyltransferase family, and is a mitochondrial acyltransferase which transfers the acyl group to the N terminus of glycine. It can conjugate a multitude of substrates to form a variety of N acylglycines.