Overview

  • Product name
    Anti-Glypican 3 antibody [SP86]
    See all Glypican 3 primary antibodies
  • Description
    Rabbit monoclonal [SP86] to Glypican 3
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from C terminal of Human Glypican 3.

  • Positive control
    • Human liver cancer tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab95363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval (boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min).

Target

  • Function
    Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
  • Tissue specificity
    Highly expressed in lung, liver and kidney.
  • Involvement in disease
    Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
  • Sequence similarities
    Belongs to the glypican family.
  • Cellular localization
    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • DGSX antibody
    • Glypican proteoglycan 3 antibody
    • Glypican-3 [Precursor] antibody
    • Gpc3 antibody
    • GPC3_HUMAN antibody
    • GTR2 2 antibody
    • GTR2-2 antibody
    • Heparan sulphate proteoglycan antibody
    • Intestinal protein OCI 5 antibody
    • Intestinal protein OCI-5 antibody
    • MXR7 antibody
    • OCI 5 antibody
    • OCI-5 antibody
    • OCI5 antibody
    • SDYS antibody
    • Secreted glypican-3 antibody
    • SGB antibody
    • SGBS antibody
    • SGBS1 antibody
    see all

Images

  • Immunohistochemical staining of human liver hepatocellular carcinoma with ab95363.

  • Flow cytometric analysis of rabbit anti-Glypican 3 (SP86) antibody ab98363 (1/100) in HEPG2 cellls (green) compared to negative control of rabbit IgG (blue).

  • ab95363, at 1/100 dilution, staining Glypican 3 in formalin-fixed, paraffin-embedded Human liver cancer tissue by Immunohistochemistry.
  • ICC/IF image of ab95363 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95363 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab95363 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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